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Overview

  • Product nameAnti-pan-AKT antibodySee all pan-AKT primary antibodies ...
  • Description
    Rabbit polyclonal to pan-AKT
  • Tested applicationsICC/IF, WB more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human pan-AKT.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab94827.)

  • Positive controlThis antibody gave a positive signal in the following lysates: Human Heart Tissue; HeLa Whole Cell; MCF7 Whole Cell; HepG2 Whole Cell; NIH 3T3 Whole Cell.

Properties

Applications

Our Abpromise guarantee covers the use of ab85683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use a concentration of 5 µg/ml.
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Can be blocked with pan-AKT peptide (ab94827).

Target

  • FunctionPlays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
  • Tissue specificityExpressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
  • Involvement in diseaseDefects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
    Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
    The AGC-kinase C-terminal mediates interaction with THEM4.
  • Post-translational
    modifications    Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.

    • Target information above from: UniProt accession P31749 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      AKT1 antibodyAKT1_HUMAN antibodyAKT2 antibody
      AKT3 antibodyPKB alpha antibodyPKB antibodyPKB beta antibodyPKBalpha antibodyPRKBA antibodyPRKBB antibodyPRKBG antibodyProtein kinase B antibodyProtein kinase B beta antibodyProtein kinase B gamma antibodyProto-oncogene c-Akt antibodyRAC alpha antibodyRAC antibodyRAC gamma antibodyRAC PK alpha antibodyRAC PK beta antibodyRAC PK gamma antibodyRAC-alpha serine/threonine-protein kinase antibodyRAC-PK-alpha antibodySTK2 antibody
    see all

Anti-pan-AKT antibody images

  • Western blot - pan-AKT antibody (ab85683)
    Western blot - pan-AKT antibody (ab85683)
    All lanes : Anti-pan-AKT antibody (ab85683) at 1 µg/ml

    Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa


    Exposure time : 8 minutes
  • Immunocytochemistry/ Immunofluorescence - pan-AKT antibody (ab85683)
    Immunocytochemistry/ Immunofluorescence - pan-AKT antibody (ab85683)
    ICC/IF image of ab85683 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85683, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Western blot - Anti-pan-AKT antibody (ab85683)
    Western blot - Anti-pan-AKT antibody (ab85683)
    Anti-pan-AKT antibody (ab85683) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa


    Exposure time : 20 minutes

References for Anti-pan-AKT antibody (ab85683)

ab85683 has not yet been referenced specifically in any publications.

Publishing research using ab85683 ? Please let us know so that we can cite the reference in this datasheet

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Product code: 85683

Inquiry: I would like to purchase Anti Akt antibody for WB in rat heart tissues. Kindly explain what do you mean Pan( Anti-pan-AKT)? Does it recognize all forms of Akt such as 1,2 &3

Thank you very much for your interest in ab85683.
I can confirm that ab85683 is not distinguishing between the different, closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) but binding all of them.
To our knowledge, ab85683 h...

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