Products:Neuroscience >> Neurology process >> Neural Signal Transduction
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ab8805 has been referenced in 7 publications.
Publishing research using ab8805? Please let us know so that we can cite the reference in this datasheet
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Predicted band size : 56 kDa
Western blot using Akt antibody ab8805 at 1/500, Goat anti-rabbit IgG peroxidase conjugate at 1/10,000. 20 ug NIH/3T3 whole cell lysate, Gel type 10% NuPage with MOPS buffer developed with Substrate Pierce SuperSignal™ West Pico
The Akt antibody (ab8805) is used at 1/80 on cultured neonatal rat cardiomyocytes that express a nuclear-targeted Akt construct. The green is Akt antibody, the red is Texas-red™ phalloidin that labels actin filaments.
Mark Sussman, Cincinnati, USA
A: Normal colon tissue
B: Tumour tissue
Akt antibody (ab8805) used at 1/1000 on formalin-fixed paraffin embedded tissue.
Anti-pan-AKT antibody (ab8805) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)
Exposure time : 6 minutes
This image is a courtesy of Anonymous Abreview
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