Overview

  • Product nameAnti-pan-AKT antibodySee all pan-AKT primary antibodies ...
  • Description
    Rabbit polyclonal to pan-AKT
  • SpecificityThe sequence used to generate this antibody, has a high degree of similarity to regions found in AKT1, AKT2 and AKT3, and thus may cross react with all of these proteins.
  • Tested applicationsICC, IHC-P, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide:

    CRPHFPQFSYSASGTA

    conjugated to KLH, corresponding to amino acids 466-480 of Human pan-AKT (100% similar to Rat, Chicken and Mouse AKT sequences). (Peptide available as ab9041.)

  • Positive control
    • WB: NIH/3T3 whole cell lysate IHC: colon tumour sections

Properties

Applications

Our Abpromise guarantee covers the use of ab8805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/80.
IHC-P 1/1000 - 1/1500.
WB 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).Can be blocked with AKT1 peptide (ab9041).
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 24759991

Target

  • FunctionPlays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
  • Tissue specificityExpressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
  • Involvement in diseaseDefects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
    Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
    The AGC-kinase C-terminal mediates interaction with THEM4.
  • Post-translational
    modifications
    Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AKT 1 antibody
    • AKT 2 antibody
    • AKT 3 antibody
    • AKT1 antibody
    • AKT1_HUMAN antibody
    • AKT2 antibody
    • AKT3 antibody
    • C AKT antibody
    • CAKT antibody
    • DKFZP434N0250 antibody
    • MGC99656 antibody
    • Murine thymoma viral (v akt) homolog 2 antibody
    • murine thymoma viral (v akt) oncogene homolog 1 antibody
    • Murine thymoma viral (vakt) oncogene homolog 1 antibody
    • PKB Akt antibody
    • PKB alpha antibody
    • PKB antibody
    • PKB beta antibody
    • PKBalpha antibody
    • PKBBETA antibody
    • PKBG antibody
    • PRKBA antibody
    • PRKBB antibody
    • PRKBG antibody
    • Protein kinase Akt 2 antibody
    • Protein kinase Akt 3 antibody
    • Protein kinase B antibody
    • Protein kinase B beta antibody
    • Protein kinase B gamma antibody
    • Proto-oncogene c-Akt antibody
    • RAC alpha antibody
    • RAC alpha serine/threonine protein kinase antibody
    • RAC antibody
    • RAC beta antibody
    • RAC beta serine/threonine protein kinase antibody
    • RAC gamma antibody
    • RAC gamma serine/threonine protein kinase antibody
    • RAC PK alpha antibody
    • RAC PK beta antibody
    • RAC PK gamma antibody
    • Rac protein kinase alpha antibody
    • Rac protein kinase beta antibody
    • RAC Serine/Threonine Protein Kinase antibody
    • RAC-alpha serine/threonine-protein kinase antibody
    • RAC-PK-alpha antibody
    • Serine threonine protein kinase Akt 3 antibody
    • STK 2 antibody
    • STK2 antibody
    • V AKT Murine Thymoma Viral Oncogene Homolog 1 antibody
    • v akt murine thymoma viral oncogene homolog 2 antibody
    • v akt murine thymoma viral oncogene homolog 3 antibody
    • VAKT Murine Thymona Viral Oncogene 1 antibody
    see all

Anti-pan-AKT antibody images



  • Predicted band size : 56 kDa


    Western blot using Akt antibody ab8805 at 1/500, Goat anti-rabbit IgG peroxidase conjugate at 1/10,000. 20 ug NIH/3T3 whole cell lysate, Gel type 10% NuPage with MOPS buffer developed with Substrate Pierce SuperSignal™ West Pico
  • The Akt antibody (ab8805) is used at 1/80 on cultured neonatal rat cardiomyocytes that express a nuclear-targeted Akt construct. The green is Akt antibody, the red is Texas-red™ phalloidin that labels actin filaments.

  • A: Normal colon tissue
    B: Tumour tissue
    Akt antibody (ab8805) used at 1/1000 on formalin-fixed paraffin embedded tissue.

  • Anti-pan-AKT antibody (ab8805) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)


    Exposure time : 6 minutes

    This image is a courtesy of Anonymous Abreview

    See Abreview

Protocols

References for Anti-pan-AKT antibody (ab8805)

This product has been referenced in:
  • Bai X  et al. Antiangiogenic treatment diminishes renal injury and dysfunction via regulation of local AKT in early experimental diabetes. PLoS One 9:e96117 (2014). IHC-FoFr, IHC-Fr ; Rat . Read more (PubMed: 24759991) »
  • Chase A  et al. Ruxolitinib as potential targeted therapy for patients with JAK2 rearrangements. Haematologica 98:404-8 (2013). WB ; Mouse . Read more (PubMed: 22875628) »

See all 11 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-15 % NuPAGE)
Sample Human Cell lysate - whole cell (Endomteriotic)
Specification Endomteriotic
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Brett McKinnon

Verified customer

Submitted Jun 10 2013

We do!

For the pan-Akt antibody we have ab4702, colon tumor slides, and ab139513 which is NIH3T3 cell lysate.

The for the Akt1 pS473 antibody ab66138 we have human breast cancer tissue slides ab46961, and TNF treated NIH3T3 lysate ab1...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information f...

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Thank you for contacting us.

I am sorry to hear you have been experiencing problems with one of our antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints. I ...

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Application Western blot
Sample Mouse Cell lysate - whole cell (macrophage cell lysate)
Loading amount 20 µg
Specification macrophage cell lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 09 2012

Thank you for contacting us. I would say that this sort of experiment is very-very tricky. We unfortunately do not have any protocol available at the moment for manually IHC staining quantification with antibodies. I would suggest ploughing through...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse embryonic stem cells)
Specification Mouse embryonic stem cells
Fixative 4% PFA
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 cell line)
Loading amount 10 µg
Specification NIH 3T3 cell line
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 13 2009

Application Western blot
Sample Human Cell lysate - whole cell (HT1080 cell line)
Loading amount 10 µg
Specification HT1080 cell line
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 13 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (breast)
Specification breast
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

Submitted Sep 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"