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Synthetic peptide: CRPHFPQFSYSASGTA conjugated to KLH, corresponding to amino acids 466-480 of Human pan-AKT (100% similar to Rat, Chicken and Mouse AKT sequences). (Peptide available as ab9041.)
Our Abpromise guarantee covers the use of ab8805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/1500.|
|WB||1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).Can be blocked with AKT1 peptide (ab9041).|
|ICC/IF||Use at an assay dependent concentration.|
The Akt antibody (ab8805) is used at 1/80 on cultured neonatal rat cardiomyocytes that express a nuclear-targeted Akt construct. The green is Akt antibody, the red is Texas-red™ phalloidin that labels actin filaments.
A: Normal colon tissue
B: Tumour tissue
Akt antibody (ab8805) used at 1/1000 on formalin-fixed paraffin embedded tissue.
This image is a courtesy of Anonymous Abreview
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"