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Anti-pan-AKT antibody (ab8805)

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Overview

Product name

Anti-pan-AKT antibody
See all pan-AKT products (6) ...

Description

Rabbit polyclonal to pan-AKT

Specificity

The sequence used to generate this antibody, has a high degree of similarity to regions found in AKT1, AKT2 and AKT3, and thus may cross react with all of these proteins.

Tested applications

ICC, IHC-P, WB, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Chicken

Immunogen

Synthetic peptide: CRPHFPQFSYSASGTA conjugated to KLH, corresponding to amino acids 466-480 of Human pan-AKT (100% similar to Rat, Chicken and Mouse AKT sequences). (Peptide available as ab9041.)

CRPHFPQFSY SASGTA

Positive control

WB: NIH/3T3 whole cell lysate IHC: colon tumour sections

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

0.02 K Phosphate, 0.15M NaCl, 0.1% Sodium Azide, pH7.2

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

  • Western blot - pan-AKT antibody (ab8805)Western blot - pan-AKT antibody (ab8805) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - pan-AKT antibody (ab8805)Immunocytochemistry/ Immunofluorescence - pan-AKT antibody (ab8805) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan-AKT antibody (ab8805)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan-AKT antibody (ab8805) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab8805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphoylate and inactivate several targets, including Bad, Forkhead transcription factors, and caspase 9. Activity of AKT has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to AKT activity, however phosphorylation of T308 has been shown to be absolutely essential for AKT activation.

Cellular localization

Cell Membrane and Cytoplasmic

Alternative names

  • AKT 1 antibody
  • AKT 2 antibody
  • AKT 3 antibody
  • AKT1 antibody
  • AKT2 antibody
  • AKT3 antibody
  • C AKT antibody
  • CAKT antibody
  • DKFZP434N0250 antibody
  • MGC99656 antibody
  • Murine thymoma viral (v akt) homolog 2 antibody
  • murine thymoma viral (v akt) oncogene homolog 1 antibody
  • Murine thymoma viral (vakt) oncogene homolog 1 antibody
  • PKB Akt antibody
  • PKB alpha antibody
  • PKB antibody
  • PKB beta antibody
  • PKBalpha antibody
  • PKBBETA antibody
  • PKBG antibody
  • PRKBA antibody
  • PRKBB antibody
  • PRKBG antibody
  • Protein kinase Akt 2 antibody
  • Protein kinase Akt 3 antibody
  • Protein kinase B antibody
  • Protein kinase B beta antibody
  • Protein kinase B gamma antibody
  • RAC alpha antibody
  • RAC alpha serine/threonine protein kinase antibody
  • RAC antibody
  • RAC beta antibody
  • RAC beta serine/threonine protein kinase antibody
  • RAC gamma antibody
  • RAC gamma serine/threonine protein kinase antibody
  • RAC PK alpha antibody
  • RAC PK beta antibody
  • RAC PK gamma antibody
  • Rac protein kinase alpha antibody
  • Rac protein kinase beta antibody
  • RAC Serine/Threonine Protein Kinase antibody
  • Serine threonine protein kinase Akt 3 antibody
  • STK 2 antibody
  • STK2 antibody
  • V AKT Murine Thymoma Viral Oncogene Homolog 1 antibody
  • v akt murine thymoma viral oncogene homolog 2 antibody
  • v akt murine thymoma viral oncogene homolog 3 antibody
  • VAKT Murine Thymona Viral Oncogene 1 antibody
see all

Anti-pan-AKT antibody images:

  Western blot - pan-AKT antibody (ab8805)

Western blot - pan-AKT antibody (ab8805)



Predicted band size : 56 kDa


Western blot using Akt antibody ab8805 at 1/500, Goat anti-rabbit IgG peroxidase conjugate at 1/10,000. 20 ug NIH/3T3 whole cell lysate, Gel type 10% NuPage with MOPS buffer developed with Substrate Pierce SuperSignal™ West Pico

  Immunocytochemistry/ Immunofluorescence - pan-AKT antibody (ab8805)

Immunocytochemistry/ Immunofluorescence - pan-AKT antibody (ab8805)

The Akt antibody (ab8805) is used at 1/80 on cultured neonatal rat cardiomyocytes that express a nuclear-targeted Akt construct. The green is Akt antibody, the red is Texas-red™ phalloidin that labels actin filaments.

Mark Sussman, Cincinnati, USA

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan-AKT antibody (ab8805)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan-AKT antibody (ab8805)

A: Normal colon tissue
B: Tumour tissue
Akt antibody (ab8805) used at 1/1000 on formalin-fixed paraffin embedded tissue.

  - AKT antibody (ab8805)

- AKT antibody (ab8805)

  Western blot - pan-AKT antibody (ab8805)

Western blot - pan-AKT antibody (ab8805)

Anti-pan-AKT antibody (ab8805) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg

Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 56 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)


Exposure time : 6 minutes

This image is a courtesy of Anonymous Abreview

See Abreview

Protocols

  • AKT IHC
  • References for Anti-pan-AKT antibody (ab8805)

    This product has been referenced in:

    • Nakou Met al. Gene network analysis of bone marrow mononuclear cells reveals activation of multiple kinase pathways in human systemic lupus erythematosus. PLoS One 5:e13351 (2010). WB; Mouse.Read more (PubMed: 20976278) »
    • Han JWet al. Impaired PI3K/Akt signal pathway and hepatocellular injury in high-fat fed rats. World J Gastroenterol 16:6111-8 (2010). WB; Rat.Read more (PubMed: 21182226) »

    See all 7 publications for this product

    Publishing research using ab8805? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"