Anti-pan-AKT (phospho T308) antibody (ab38449)

Overview

  • Product nameAnti-pan-AKT (phospho T308) antibody
    See all pan-AKT primary antibodies
  • Description
    Rabbit polyclonal to pan-AKT (phospho T308)
  • Specificityab38449 recognises endogenous levels of AKT (pT308), AKT2 (pT309) and AKT3 (pT305) only when phosphorylated at those residues.
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 308 (MKTpFC).

  • Positive control
    • Human lung carcinoma, extracts from 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab38449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionPlays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
  • Tissue specificityExpressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
  • Involvement in diseaseDefects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
    Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
    The AGC-kinase C-terminal mediates interaction with THEM4.
  • Post-translational
    modifications
    Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
    Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AKT1 antibody
    • AKT1_HUMAN antibody
    • AKT2 antibody
    • AKT3 antibody
    • MGC9965 antibody
    • Oncogene AKT1 antibody
    • PKB alpha antibody
    • PKB antibody
    • PKB beta antibody
    • PKBalpha antibody
    • PRKBA antibody
    • PRKBB antibody
    • PRKBG antibody
    • Protein kinase B antibody
    • Protein kinase B beta antibody
    • Protein kinase B gamma antibody
    • Proto-oncogene c-Akt antibody
    • RAC alpha antibody
    • RAC antibody
    • RAC gamma antibody
    • RAC PK alpha antibody
    • RAC PK beta antibody
    • RAC PK gamma antibody
    • RAC-alpha serine/threonine-protein kinase antibody
    • RAC-PK-alpha antibody
    • STK2 antibody
    see all

Anti-pan-AKT (phospho T308) antibody images

  • All lanes : Anti-pan-AKT (phospho T308) antibody (ab38449) at 1/500 dilution

    Lane 1 : The extracts from 293 cells untreated
    Lane 2 : The extracts from 293 cells treated with insulin.

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Immunohistochemical analysis of AKT (phospho T308) expression in paraffin embedded human lung carcinoma tissue, using ab38449 (1/50). Right-hand panel represents a negative control where ab38449 was pre-incubated with the immunizing (blocking) peptide.

References for Anti-pan-AKT (phospho T308) antibody (ab38449)

This product has been referenced in:
  • Nteeba J  et al. Impact of obesity on ovotoxicity induced by 7,12-dimethylbenz[a]anthracene in mice. Biol Reprod 90:68 (2014). WB ; Mouse . Read more (PubMed: 24501177) »
  • Agbemenyah HY  et al. Insulin growth factor binding protein 7 is a novel target to treat dementia. Neurobiol Dis N/A:N/A (2013). WB ; Mouse, Human . Read more (PubMed: 24075854) »

See all 8 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Baboon Tissue lysate - other (Fetal skeletal muscle)
Specification Fetal skeletal muscle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Soon-ok Kim

Verified customer

Submitted Dec 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Purified protein (Human primary CD4 T cells)
Specification Human primary CD4 T cells
Treatment TCR activated using anti-CD3/CD28 for 3,10 and 30min
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Marco Craveiro

Verified customer

Submitted Jul 23 2013


We do have a blocking peptide available for ab38449 however this product is made to order, so it would take approximately 2 months for the order to be processed and shipped.
Please let me know if you would be interested in this product and if...

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Thank you for your reply.
You should receive ab8933 tomorrow morning and your order number (for tracking purposes only) is *. If you have any issues with the new antibody, or if there is anything else I can help you with, please let me know.

Thank you for your reply.
As ab8933, is covered under our Abpromise to work in western blotting on human samples, then we will replace or refund the cost of the antibody of it fails to work for you.
Please let me know if you would like to try a...

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Thank you for contacting Abcam.
I am sorry about the issues that you are having with ab38449 in western blotting. Could the band that is in your image you sent at about 55kDa be the correct band? I know it is not in all your plus insulin lanes, so ...

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Thank you for your reply. I have reviewed the customer's comments and image and agree that this antibody is not working as stated on the datasheet.  I am happy to offer a replacement or credit.  Please let me know which the customer would prefer. ...

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Thank you for contacting Abcam. We would be very happy if your customer tested ab38449 in ICC/IF in mouse cells. I have placed the discount code and instructions below. If there is anything else I can help you with please let me know. DISCOUNT CODE: **...

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Thank you for contacting Abcam. I am sorry that your customer is experiencing difficulties with this antibody in WB. I have reviewed the protocol information provided and would like to make some suggestions to improve the results with this product....

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Thank you for your enquiry. I have contacted the originator of the product but unfortunately, the exact peptide sequence information is proprietary. However, based on the sequence we found for Entamoeba histolytica (swiss prot#Q761W9: Putative uncha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"