Anti-pan-AKT (phospho T308) antibody (ab8933)

Overview

• Product nameAnti-pan-AKT (phospho T308) antibodySee all pan-AKT primary antibodies ...
• Description
  Rabbit polyclonal to pan-AKT (phospho T308)
• SpecificityThis antibody was made against a peptide directed against the phosphorylated form of AKT1 at T308, but due to a high degree of homology it is predicted to cross react with AKT2 and AKT3 if they are phosphorylated at the corresponding residue.
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide: DGATMKT_pFCG conjugated to KLH, corresponding to amino acids 302-311 of AKT.

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Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage buffer0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Heart disease

  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Serine/threonine kinases
  • AKT

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • PKB / AKT

  • Cardiovascular
  • Atherosclerosis
  • Apoptosis

  • Cardiovascular
  • Heart
  • Cardiac metabolism

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Apoptosis
  • Nuclear

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

Applications

Target

• FunctionPlays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
• Tissue specificityExpressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
• Involvement in diseaseDefects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
  Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
  Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 1 PH domain.
  Contains 1 protein kinase domain.
• DomainBinding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
  The AGC-kinase C-terminal mediates interaction with THEM4.
• Post-translational
  modificationsPhosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
  Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
• Cellular localizationCytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.

Target information above from: UniProt accession P31749 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 207 Human
  • Omim: 164730 Human
  • SwissProt: P31749 Human
  • Unigene: 525622 Human

• Alternative names
  AKT 1 antibodyAKT 2 antibodyAKT 3 antibody

  AKT1 antibodyAKT1_HUMAN antibodyAKT2 antibodyAKT3 antibodyC AKT antibodyCAKT antibodyDKFZP434N0250 antibodyMGC99656 antibodyMurine thymoma viral (v akt) homolog 2 antibodymurine thymoma viral (v akt) oncogene homolog 1 antibodyMurine thymoma viral (vakt) oncogene homolog 1 antibodyPKB Akt antibodyPKB alpha antibodyPKB antibodyPKB beta antibodyPKBalpha antibodyPKBBETA antibodyPKBG antibodyPRKBA antibodyPRKBB antibodyPRKBG antibodyProtein kinase Akt 2 antibodyProtein kinase Akt 3 antibodyProtein kinase B antibodyProtein kinase B beta antibodyProtein kinase B gamma antibodyProto-oncogene c-Akt antibodyRAC alpha antibodyRAC alpha serine/threonine protein kinase antibodyRAC antibodyRAC beta antibodyRAC beta serine/threonine protein kinase antibodyRAC gamma antibodyRAC gamma serine/threonine protein kinase antibodyRAC PK alpha antibodyRAC PK beta antibodyRAC PK gamma antibodyRac protein kinase alpha antibodyRac protein kinase beta antibodyRAC Serine/Threonine Protein Kinase antibodyRAC-alpha serine/threonine-protein kinase antibodyRAC-PK-alpha antibodySerine threonine protein kinase Akt 3 antibodySTK 2 antibodySTK2 antibodyV AKT Murine Thymoma Viral Oncogene Homolog 1 antibodyv akt murine thymoma viral oncogene homolog 2 antibodyv akt murine thymoma viral oncogene homolog 3 antibodyVAKT Murine Thymona Viral Oncogene 1 antibody

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Anti-pan-AKT (phospho T308) antibody images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-pan-AKT (phospho T308) antibody(ab8933)
  ab8933 (4µg/ml) staining AKT (phospho T308) in human breast using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane and cytoplasmic compartment within the breast ductal regions
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.