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Product name
Anti-pan Arrestin antibody
Description
Rabbit polyclonal to pan Arrestin
Specificity
Ab2914 detects recombinant rat beta-arrestin and beta-arrestin2 (not tested against endogenous protein). This antibody does not detect visual or cone arrestin.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic Peptide: CD(384)DIVFEDFARLRLK(397). Immunizing peptide corresponds to amino acid residues 384-397 from human beta-arrestin2.
C-DDIVFEDFARLRLK
Positive control
COS7 cell lysates overexpressing rat beta arrestin 2 (flag tagged)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Arrestins are cytosolic proteins that are involved in G protein-coupled receptor (GPCR) desensitization. Arrestin binding to activated GPCRs is phosphorylation dependent and, once bound, uncouple the GPCR from the associated heterotrimeric G proteins. There are currently 4 known mammalian isoforms, beta-arrestin1 (arrestin2), beta-arrestin2 (arrestin3), visual arrestin (arrestin1), and cone arrestin. The beta- isoforms are ubiquitously expressed and are known to interact with acetylcholine and adrenergic receptors. Visual and cone arrestins are found to interact directly with transducin.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> Regulators
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
Western Blot: use at 3 µg/ml.
By Western blot, this antibody detects ~49 kDa and ~47 kDa proteins representing recombinant beta-arrestin and beta-arrestin2, respectively.
Target
Relevance
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors.
Cellular localization
Cytoplasmic and Plasma membrane
Alternative names
- ARB1 antibody
- ARB2 antibody
- ARR1 antibody
see all
Database links
- Entrez Gene: 408 Human
- Entrez Gene: 409 Human
- Omim: 107940 Human
- Omim: 107941 Human
- SwissProt: P49407 Human
- SwissProt: P32121 Human
Anti-pan Arrestin antibody images:
Immunocytochemistry/ Immunofluorescence - pan Arrestin antibody (ab2914)
ab2914 staining human HEK 293 cells by ICC/IF. The cells were fixed in 4% PFA (in PBS) for 30min. After washing the cells were permeabilised with 0.01% Triton X-100 and then incubated with the primary antibody in PBS-T for 18h at 4C. An Alexa Fluor ® 488 conjugated donkey polyclonal antibody was used as the secondary (staining shown in green). After three 5min washes with PBS the cover slips were mounted with slow fade mounting solution containing DAPI (blue) .
This image is courtesy of an Abreview submitted by Mrs Danijela Markovic
Western blot - pan Arrestin antibody (ab2914)
Anti-pan Arrestin antibody (ab2914) at 1 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 49 +51 kDa (why is the actual band size different from the predicted?)
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 90 seconds
pan Arrestin contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References for Anti-pan Arrestin antibody (ab2914)
This product has been referenced in:
- Law IK et al. Neurotensin-induced pro-inflammatory signaling in human colonocytes is regulated by beta-arrestins and endothelin-converting enzyme-dependent endocytosis and re-sensitization of NT receptor 1. J Biol Chem : (2012).Read more (PubMed: 22416137) »
- Kuhr FK et al. Beta-arrestin 2 is required for B1 receptor-dependent post-translational activation of inducible nitric oxide synthase. FASEB J 24:2475-83 (2010). WB; Human.Read more (PubMed: 20228252) »
See all 7 publications for this product
Publishing research using ab2914? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"