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Products:Immunology >> Immunoglobulins >> Receptors
Overview
Product name
Anti-pan CEACAM antibody [D14HD11]
See all pan CEACAM products (2) ...
Description
Mouse monoclonal [D14HD11] to pan CEACAM
Specificity
Broadly reactive with high affinity (affinity to solid phase bound CEACAM 5: 2 x 1010 L/Mol [Jantscheff P et al.]) with most of the CEACAM molecules (except CEACAM 7 and CEACAM 8) transiently expressed on the cell surface of transfected BOSC23 cells. This antibody can be used to detect CEACAM 1 (BGP/CD66a), CEACAM 3 (CGM1/CD66d), CEACAM 4 (CGM 7), CEACAM 5 and CEACAM 6 (NCA/CD66c) on transfectants by flow cytometry.
Tested applications
Flow Cyt, ELISA, IHC-Fr, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length native Carcino Embryonic Antigen (partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Jantscheff Pet al.).
General notes
Antibodies produced from cDNA:
Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein.
Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself.
Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
D14HD11
Isotype
IgG1
Research areas
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Immunoglobulins >> Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-P
IHC-P: 1/40IHC-P: 1/40
WB
WB: 1/40WB: 1/40
Target
Relevance
CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
Cellular localization
Cell Membrane
Alternative names
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
see all
Database links
- Entrez Gene: 1048 Human
- Entrez Gene: 1084 Human
- Entrez Gene: 1089 Human
- Entrez Gene: 4680 Human
- Entrez Gene: 634 Human
- SwissProt: O75871 Human
- SwissProt: P06731 Human
- SwissProt: P40198 Human
see all
References for Anti-pan CEACAM antibody [D14HD11] (ab4567)
This product has been referenced in:
- Green LRet al. Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells. Infect Immun 79:2241-9 (2011).Read more (PubMed: 21464080) »
See 1 publication for this product
Publishing research using ab4567? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"