pan Caspase (active) FITC Staining Kit (ab65611)

Overview

  • Product namepan Caspase (active) FITC Staining Kit
    See all Caspase kits
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Fluorescein Caspase Staining Kit provides a convenient and sensitive means for detecting activated caspases in living cells. The assay utilizes the caspase family inhibitor VAD-FMK conjugated to FITC (FITC-VAD-FMK) as a marker. FITC-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of caspases plays a central role in apoptosis.

  • Tested applicationsFluorescence Microscopy, Functional Studies, Flow Cytmore details

Properties

  • RelevanceCaspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • Cellular localizationCytoplasmic

Applications

Our Abpromise guarantee covers the use of ab65611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Fluorescence Microscopy Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

Protocols

References for pan Caspase (active) FITC Staining Kit (ab65611)

ab65611 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"