pan Cytokeratin antibody [5D3 + LP34] (ab17153)

Product Name 

pan Cytokeratin antibody [5D3 + LP34]

Product type 

Primary antibodies

Description 

Mouse monoclonal [5D3 + LP34] to pan Cytokeratin

Immunogen 

Cytokeratins from human breast carcinoma cell line MCF-7 and detergent insoluble fraction of psoriatic epidermis.

Reacts with 
(species key)

Hu

Specificity 

This antibody stains cytokeratin 5,6,8 and 18 and has been designed for a broad spectrum screening of cytokeratins. It stains carcinomas of simple and squamous origin.

Tested applications 
(see key)

ICC/IF, IHC-P

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/15 - 1/50 (an incubation period of 30 minutes at room temperature is recommended). Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Skin.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins

Relevance 

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors.

There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

5D3 + LP34

Isotype 

IgG

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Storage instructions 

Store at +4°C.

Notes 

This antibody is a cocktail of two different clones; 5D3 (IgG2a) and LP53 (IgG1).

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