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Anti-pan Cytokeratin antibody [5D3 + LP34] (ab17153)

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Overview

Product name

Anti-pan Cytokeratin antibody [5D3 + LP34]
See all pan Cytokeratin products (18) ...

Description

Mouse monoclonal [5D3 + LP34] to pan Cytokeratin

Specificity

This antibody stains cytokeratin 5,6,8 and 18 and has been designed for a broad spectrum screening of cytokeratins. It stains carcinomas of simple and squamous origin.

Tested applications

ICC/IF, IHC-P, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Cytokeratins from human breast carcinoma cell line MCF-7 and detergent insoluble fraction of psoriatic epidermis.

Positive control

Skin.

General notes

This antibody is a cocktail of two different clones; 5D3 (IgG2a) and LP53 (IgG1).

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

5D3 + LP34

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence-pan Cytokeratin antibody [5D3 + LP34](ab17153)Immunocytochemistry/ Immunofluorescence-pan Cytokeratin antibody [5D3 + LP34](ab17153) image (enlarge)

  • Western blot - pan Cytokeratin antibody [5D3 + LP34] (ab17153)Western blot - pan Cytokeratin antibody [5D3 + LP34] (ab17153) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab17153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

Cellular localization

Cytoplasmic

Anti-pan Cytokeratin antibody [5D3 + LP34] images:

  Immunocytochemistry/ Immunofluorescence-pan Cytokeratin antibody [5D3 + LP34](ab17153)

Immunocytochemistry/ Immunofluorescence-pan Cytokeratin antibody [5D3 + LP34](ab17153)

ICC/IF image of ab17153 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Western blot - pan Cytokeratin antibody [5D3 + LP34] (ab17153)

Western blot - pan Cytokeratin antibody [5D3 + LP34] (ab17153)

Anti-pan Cytokeratin antibody [5D3 + LP34] (ab17153) at 1/500 dilution ((in 5% Marvel TBS-T). Incubation for 1 hour at 22°C) + Whole cell lysate of human HT1080 cells at 25 µg

Secondary
An HRP-conjugated Sheep anti-mouse IgG monoclonal at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Observed band size : 45 kDa (why is the actual band size different from the predicted?)


Exposure time : 15 minutes

Blocking Step: 5% Milk for 1 hour at 22°C

This image is courtesy of an anonymous Abreview

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References for Anti-pan Cytokeratin antibody [5D3 + LP34] (ab17153)

ab17153 has not yet been referenced specifically in any publications.

Publishing research using ab17153? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"