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Immunocytochemistry/ Immunofluorescence-pan Cytokeratin antibody [5D3 + LP34](ab17153)
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Product Name
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pan Cytokeratin antibody [5D3 + LP34]
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See all pan Cytokeratin antibodies (18)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [5D3 + LP34] to pan Cytokeratin
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Immunogen
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Cytokeratins from human breast carcinoma cell line MCF-7 and detergent insoluble fraction of psoriatic epidermis.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody stains cytokeratin 5,6,8 and 18 and has been designed for a broad spectrum screening of cytokeratins. It stains carcinomas of simple and squamous origin.
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Tested applications
(see key)
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ICC/IF, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions IHC-P: 1/15 - 1/50 (an incubation period of 30 minutes at room temperature is recommended). Perform enzymatic antigen retrieval before commencing with IHC staining protocol. ICC/IF: Use at a concentration of 1 - 5 µg/ml.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Skin.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
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Relevance
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Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors.
There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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5D3 + LP34
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Isotype
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IgG
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Storage buffer
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Preservative: None Constituents: PBS
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Form
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Liquid
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Storage instructions
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Store at +4°C.
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Notes
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This antibody is a cocktail of two different clones; 5D3 (IgG2a) and LP53 (IgG1).
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this pan Cytokeratin antibody [5D3 + LP34] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for pan Cytokeratin antibody [5D3 + LP34] images, references, products related to ab17153 and other tools.
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pan Cytokeratin antibody [5D3 + LP34] images:
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Immunocytochemistry/ Immunofluorescence-pan Cytokeratin antibody [5D3 + LP34](ab17153)
ICC/IF image of ab17153 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to pan Cytokeratin
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pan Cytokeratin antibody [5D3 + LP34] - more information
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