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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Overview
Product name
Anti-pan Cytokeratin antibody [5D3 & LP34], prediluted
See all pan Cytokeratin products (18) ...
Description
Mouse monoclonal [5D3 & LP34] to pan Cytokeratin, prediluted
Specificity
This antibody is a cocktail of two different clones; 5D3 and LP53. This antibody stains cytokeratin 5,6,8 and 18. This antibody has been designed for a broad spectrum screening of cytokeratins. It stains carcinomas of simple and squamous origin.
Tested applications
IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Cytokeratins from human breast carcinoma cell line MCF-7 and detergent insoluble fraction of psoriatic epidermis.
General notes
Isotype IgG2a and IgG1
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Inert stabilizer
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
5D3 & LP34
Myeloma
unknown
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-Fr
Application notes
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Proteolytic digestion of tissue sections is required prior to immunostaining.
Target
Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Cellular localization
Cytoplasmic
References for Anti-pan Cytokeratin antibody [5D3 & LP34], prediluted (ab848)
ab848 has not yet been referenced specifically in any publications.
Publishing research using ab848? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"