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Anti-pan Cytokeratin antibody [7H8C4] (ab66387)

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Overview

Product name

Anti-pan Cytokeratin antibody [7H8C4]
See all pan Cytokeratin products (18) ...

Description

Mouse monoclonal [7H8C4] to pan Cytokeratin

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Ni-NTA purified truncated recombinant Cytokeratin expressed in E. Coli strain BL21 (DE3)

Positive control

Human lung squamous cell carcinoma. Human hepatocyte. Human colon adenocarcinoma. Human stomach tissue.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.03% Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

7H8C4

Isotype

IgG1

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins

  • Western blot - pan Cytokeratin antibody [7H8C4] (ab66387)Western blot - pan Cytokeratin antibody [7H8C4] (ab66387) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab66387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    WB

     WB: 1/500 - 1/2000.

    WB: 1/500 - 1/2000.

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

  • 4 Images

    IHC-P

     IHC-P: 1/500 - 1/2000.

    IHC-P: 1/500 - 1/2000.

Target

Relevance

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

Cellular localization

Cytoplasmic

Anti-pan Cytokeratin antibody [7H8C4] images:

  Western blot - pan Cytokeratin antibody [7H8C4] (ab66387)

Western blot - pan Cytokeratin antibody [7H8C4] (ab66387)

All lanes : Anti-pan Cytokeratin antibody [7H8C4] (ab66387) at 1/500 dilution

Lane 1 : Molecular weight markers
Lane 2 : Truncated cytokeratin recombinant protein

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue using ab66387 at a dilution of 1/500 - 1/2000 with DAB staining.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387)

Immunohistochemical analysis of paraffin-embedded human normal stomach tissue using ab66387 at a dilution of 1/500 - 1/2000 with DAB staining.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387)

Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue using ab66387 at a dilution of 1/500 - 1/2000 with DAB staining.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [7H8C4] (ab66387)

Immunohistochemical analysis of paraffin-embedded human normal hepatocyte tissue using ab66387 at a dilution of 1/500 - 1/2000 with DAB staining.

References for Anti-pan Cytokeratin antibody [7H8C4] (ab66387)

ab66387 has not yet been referenced specifically in any publications.

Publishing research using ab66387? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"