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Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)

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Overview

Product name

Anti-pan Cytokeratin antibody [AE1+AE3], prediluted
See all pan Cytokeratin products (18) ...

Description

Mouse monoclonal [AE1+AE3] to pan Cytokeratin, prediluted

Specificity

This antibody mixture recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52 kDa.

Tested applications

ICC/IF, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Fish

Immunogen

Human epidermal cytokeratins

Positive control

Skin or adenocarcinoma.

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

PBS pH7.3 with protein carrier and preservative

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

AE1+AE3

Isotype

IgG1

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins

Applications

Show applications key

Our Abpromise guarantee covers the use of ab961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

Cellular localization

Cytoplasmic

References for Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)

This product has been referenced in:

  • Laviolette LAet al. 17beta-estradiol accelerates tumor onset and decreases survival in a transgenic mouse model of ovarian cancer. Endocrinology 151:929-38 (2010). IHC-P; Mouse.Read more (PubMed: 20056833) »
  • Wu Xet al. Opposing roles for calcineurin and ATF3 in squamous skin cancer. Nature 465:368-72 (2010). Human.Read more (PubMed: 20485437) »

See all 9 publications for this product

Publishing research using ab961? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"