pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)

Product Name 

pan Cytokeratin antibody [AE1+AE3], prediluted

Product type 

Primary antibodies

Description 

Mouse monoclonal [AE1+AE3] to pan Cytokeratin, prediluted

Immunogen 

Human epidermal cytokeratins

Reacts with 
(species key)

Hu, Ms, Rat, Chk, Cow, Dog, Fsh, Rb

Specificity 

This antibody mixture recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52 kDa.

Tested applications 
(see key)

IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

This antibody is ready to use.

Enzymatic antigen retrieval is preferred but this may impair signal when using fluorescence detection methods. Reducing the incubation time or diluting the trypsin may improve the staining.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Skin or adenocarcinoma.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins

Relevance 

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors.

There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

AE1+AE3

Isotype 

IgG1

Storage buffer 

PBS pH7.3 with protein carrier and preservative

Form 

Liquid

Storage instructions 

Store at +4°C. Do not freeze.

References for pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)

  This product has been used in: (two most recent references)

Schwartz JL et al. RNA from brush oral cytology to measure squamous cell carcinoma gene expression. J Oral Pathol Med 37:70-7 (2008). PubMed: 18197850

Bunton TE The immunocytochemistry of cytokeratin in fish tissues. Vet Pathol 30:418-25 (1993). PubMed: 7505508

See all 6 publication references for this product.


If you publish research using ab961 please let us know so that we can cite the reference on this datasheet.

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