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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Anti-pan Cytokeratin antibody [C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2] (ab11213)
Overview
Product name
Anti-pan Cytokeratin antibody [C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2]
See all pan Cytokeratin products (18) ...
Description
Mouse monoclonal [C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2] to pan Cytokeratin
Specificity
ab11213 recognizes human cytokeratins 1, 4, 5, 6, 8, 10, 13, 18, and 19 in immunoblotting. It is a broad spectrum reagent which reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody mixture reacts with simple, cornifying and non-cornifying squamous epithelia and pseudo-stratified epithelia. It does not react with non-epithelial normal human tissues.
Tested applications
WB, IHC-Pmore details
Cross reactivity
The Anti-Pan Cytokeratin (mixture) exhibits a wide interspecies reactivity down to Xenopus laevis.
Immunogen
Prepared from a mixture of human cytokeratins.
General notes
Monoclonal Anti-Pan Cytokeratin (mouse IgG1 and IgG2a isotypes) is a mixture of monoclonal antibodies from the following clones: C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Whole Serum, 7% Horse serum, 10ug/ml Phenol red
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution.
IHC-P
IHC-P: 1/100IHC-P: 1/100
Target
Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Cellular localization
Cytoplasmic
References for Anti-pan Cytokeratin antibody [C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2] (ab11213)
This product has been referenced in:
- Yoshida Tet al. Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas. Genes Cells 13:667-78 (2008).Read more (PubMed: 18498355) »
See 1 publication for this product
Publishing research using ab11213? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"