pan Cytokeratin antibody [C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2] (ab11213)

Product Name 

pan Cytokeratin antibody [C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2]

Product type 

Primary antibodies

Description 

Mouse monoclonal [C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2] to pan Cytokeratin

Immunogen 

Prepared from a mixture of human cytokeratins.

Species Reactivity 

The Anti-Pan Cytokeratin (mixture) exhibits a wide interspecies reactivity down to Xenopus laevis.

Specificity 

ab11213 recognizes human cytokeratins 1, 4, 5, 6, 8, 10, 13, 18, and 19 in immunoblotting. It is a broad spectrum reagent which reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody mixture reacts with simple, cornifying and non-cornifying squamous epithelia and pseudo-stratified epithelia. It does not react with non-epithelial normal human tissues.

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100.
WB: Use at an assay dependent dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins

Relevance 

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors.

There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2

Isotype 

IgG

Purity 

Ascites

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: Whole Serum, 7% Horse serum, 10ug/ml Phenol red

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

20.000 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

Notes 

Monoclonal Anti-Pan Cytokeratin (mouse IgG1 and IgG2a isotypes) is a mixture of monoclonal antibodies from the following clones: C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2.

References for pan Cytokeratin antibody [C-11, PCK-26, CY-90, KS-1A3, M20, and A53-B/A2] (ab11213)

  This product has been used in:

Yoshida T et al. Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas. Genes Cells 13:667-78 (2008). PubMed: 18498355

If you publish research using ab11213 please let us know so that we can cite the reference on this datasheet.

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