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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Overview
Product name
Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin)
See all pan Cytokeratin products (18) ...
Description
Mouse monoclonal [C-11] to pan Cytokeratin (Phycoerythrin)
Conjugation
Phycoerythrin
Specificity
ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with all species (recognized epitope conserved within all species)
Immunogen
Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.2% BSA, PBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Clonality
Monoclonal
Clone number
C-11
Isotype
IgG1
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10-20µl for 106 cells. ((in a 100µl cell suspension).)
Target
Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Cellular localization
Cytoplasmic
References for Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin) (ab52460)
This product has been referenced in:
- Rajendran Set al. Preclinical evaluation of gene delivery methods for the treatment of loco-regional disease in breast cancer. Exp Biol Med (Maywood) 236:423-34 (2011).Read more (PubMed: 21444371) »
- Rajendran Set al. Targeting of breast metastases using a viral gene vector with tumour-selective transcription. Anticancer Res 31:1627-35 (2011).Read more (PubMed: 21617219) »
See all 3 publications for this product
Publishing research using ab52460? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"