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Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Our Abpromise guarantee covers the use of ab52460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10-20µl for 106 cells. (in a 100µl cell suspension).
ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.
Recommended conc. 0.5 μg/ml
Overlay with Isotype mouse IgG1 control.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"