Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin) (ab52460)

Overview

  • Product nameAnti-pan Cytokeratin antibody [C-11] (Phycoerythrin)
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [C-11] to pan Cytokeratin (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with all species (recognized epitope conserved within all species)
  • Immunogen

    Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPrior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
  • Clonality Monoclonal
  • Clone numberC-11
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab52460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10-20µl for 106 cells. (in a 100µl cell suspension).

Target

  • RelevanceCytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localizationCytoplasmic

References for Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin) (ab52460)

This product has been referenced in:
  • Rajendran S  et al. Preclinical evaluation of gene delivery methods for the treatment of loco-regional disease in breast cancer. Exp Biol Med (Maywood) 236:423-34 (2011). Read more (PubMed: 21444371) »
  • Rajendran S  et al. Targeting of breast metastases using a viral gene vector with tumour-selective transcription. Anticancer Res 31:1627-35 (2011). Read more (PubMed: 21617219) »

See all 3 Publications for this product

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We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:

Click here (or use the following: http://www.abcam.com/index.html?datasheet=52460).

For collagen I, we have onl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"