Anti-pan Cytokeratin antibody [C-11] (ab7753)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)

ab7753 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab7753 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Carl Hobbs

Immunocytochemistry/ Immunofluorescence - pan Cytokeratin antibody [C-11] (ab7753)

This image shows A549 cells stained with pan Cytokeratin (green) and with Hoechst33342 (blue) as counterstaining.

The picture was kindly supplied as part of the review submitted by one of our customers

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [C-11] (ab7753)

ab7753 staining pan Cytokeratin in guinae pig breast carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

Western blot - pan Cytokeratin antibody [C-11] (ab7753)

Anti-pan Cytokeratin antibody [C-11] (ab7753) + human A431 cell line on 10% gel.

This is a pan cytokeratin antibody and there are several cytokeratin bands between about 40 and 55 kDa. The identity of the particular cytokeratin that each band corresponds to has not been determined.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] (ab7753)

ab7753 staining rat embryonic skin/organ sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab77539 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Carl Hobbs