![Immunocytochemistry/ Immunofluorescence - pan Cytokeratin antibody [C-11] (ab7753) image](#ab7753_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [C-11] (ab7753) image](#ab7753_5.jpg)
![Western blot - pan Cytokeratin antibody [C-11] (ab7753) image](#pan-Cytokeratin-Primary-antibodies-ab7753-1.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Overview
Product name
Anti-pan Cytokeratin antibody [C-11]
See all pan Cytokeratin products (18) ...
Description
Mouse monoclonal [C-11] to pan Cytokeratin
Specificity
Cytokeratin peptides 4,5,6,8,10,13,18.
Tested applications
ICC/IF, Flow Cyt, ICC, IHC-P, WB, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
a wide range of other species, all Mammals
Immunogen
A keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Positive control
ICC: A549 cells (see review) WB: mouse skin cell lysate (See review)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascites using precipitation methods.
Clonality
Monoclonal
Clone number
C-11
Isotype
IgG1
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/250 - 1/500.(1/250 - 1/500. Fix with 4% PFA and permeabilize with 0.5 % Triton (See Abreview).)
Flow Cyt: Use a concentration of 0.5 µg/ml
ICC: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB: 1/500 - 1/1000.(1/500 - 1/1000 (See Abreview).)
IP: Use at an assay dependent dilution.
Target
Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Cellular localization
Cytoplasmic
Anti-pan Cytokeratin antibody [C-11] images:
Immunocytochemistry/ Immunofluorescence - pan Cytokeratin antibody [C-11] (ab7753)
![Immunocytochemistry/ Immunofluorescence - pan Cytokeratin antibody [C-11] (ab7753)](/ps/datasheet/Images/7/ab7753/ab7753_1.jpg)
This image shows A549 cells stained with pan Cytokeratin (green) and with Hoechst33342 (blue) as counterstaining.
The picture was kindly supplied as part of the review submitted by one of our customers
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [C-11] (ab7753)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [C-11] (ab7753)](/ps/datasheet/Images/7/ab7753/ab7753_5.jpg)
ab7753 staining pan Cytokeratin in guinae pig breast carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Western blot - pan Cytokeratin antibody [C-11] (ab7753)
![Western blot - pan Cytokeratin antibody [C-11] (ab7753)](/ps/datasheet/images/7/ab7753/pan-Cytokeratin-Primary-antibodies-ab7753-1.jpg)
Anti-pan Cytokeratin antibody [C-11] (ab7753) + human A431 cell line on 10% gel.
This is a pan cytokeratin antibody and there are several cytokeratin bands between about 40 and 55 kDa. The identity of the particular cytokeratin that each band corresponds to has not been determined.
References for Anti-pan Cytokeratin antibody [C-11] (ab7753)
This product has been referenced in:
- Meng LHet al. Cytokeratin-RNA cross-linking mediated by the antitumor aminoflavone, 5-amino-2,3-fluorophenyl-6,8-difluoro-7-methyl-4H-1-benzopyran-4-one. J Pharmacol Exp Ther 325:674-80 (2008).Read more (PubMed: 18287208) »
- Skibinski Get al. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am J Physiol Lung Cell Mol Physiol 293:L69-76 (2007).Read more (PubMed: 17384084) »
See all 7 publications for this product
Publishing research using ab7753? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"