pan Cytokeratin antibody [C-11] (ab7753)

Product Name 

pan Cytokeratin antibody [C-11]

Product type 

Primary antibodies

Description 

Mouse monoclonal [C-11] to pan Cytokeratin

Immunogen 

A keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Reacts with 

Hu

Predicted to react 
(species key)

Many, Mamm
(Due to sequence homology)

Specificity 

Cytokeratin peptides 4,5,6,8,10,13,18.

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: Use at an assay dependent dilution.
ICC/IF: 1/250 - 1/500. Fix with 4% PFA and permeabilize with 0.5 % Triton (See Abreview).
IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000 (See Abreview).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

ICC: A549 cells (see review)
WB: mouse skin cell lysate (See review)

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins

Relevance 

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors.

There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

C-11

Isotype 

IgG1

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites using precipitation methods.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for pan Cytokeratin antibody [C-11] images, references, products related to ab7753 and other tools.

pan Cytokeratin antibody [C-11] images:

References for pan Cytokeratin antibody [C-11] (ab7753)

  This product has been used in: (two most recent references)

Meng LH et al. Cytokeratin-RNA cross-linking mediated by the antitumor aminoflavone, 5-amino-2,3-fluorophenyl-6,8-difluoro-7-methyl-4H-1-benzopyran-4-one. J Pharmacol Exp Ther 325:674-80 (2008). PubMed: 18287208

Skibinski G et al. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am J Physiol Lung Cell Mol Physiol 293:L69-76 (2007). PubMed: 17384084

See all 7 publication references for this product.


If you publish research using ab7753 please let us know so that we can cite the reference on this datasheet.

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