pan Cytokeratin antibody [Clone C-11] (FITC) (ab11212)

Product Name 

pan Cytokeratin antibody [Clone C-11] (FITC)

Product type 

Primary antibodies

Description 

Mouse monoclonal [Clone C-11] to pan Cytokeratin (FITC)

Immunogen 

Keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431.

Species Reactivity 

ab11212 exhibits a wide interspecies reactivity down to xenopus laevis (e.g., human, bovine, rat, and frog).

Specificity 

Recognizes human cytokeratins 4,5,6,8,10,13, and 18 in immunoblotting.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/500. Fix cells with ethanol.
ICC/IF: 1/50.
WB: Use at an assay dependent dilution. See Bártek J et al. , Bártková J et al., Kasper references.
IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. See Bártek J et al. , Bártková J et al. references.
IHC-Fr: Use at an assay dependent dilution. Fix with methanol or acetone.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tested with PtK₂ cells.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins

Relevance 

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors.

There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

Clone C-11

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS (pH7.4)

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Protein A purified immunoglobulin from mouse ascites fluid is conjugated to FITC (isomer I) and then purified by gel filtration to remove free FITC.

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

Batch dependent within range: 1.40 - 1.40 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C. Do not freeze.

References for pan Cytokeratin antibody [Clone C-11] (FITC) (ab11212)

  This product has been used in: (two most recent references)

Boyd ZS et al. A tumor sorting protocol that enables enrichment of pancreatic adenocarcinoma cells and facilitation of genetic analyses. J Mol Diagn 11:290-7 (2009). Flow Cyt; Human. PubMed: 19460940

Xie H et al. Proteomics analysis of cells in whole saliva from oral cancer patients via value-added three-dimensional peptide fractionation and tandem mass spectrometry. Mol Cell Proteomics 7:486-98 (2008). ICC/IF; Human. PubMed: 18045803

See all 8 publication references for this product.


If you publish research using ab11212 please let us know so that we can cite the reference on this datasheet.

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