Specific protocols
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To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you get on. |
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General protocols
Useful resources:Western blotting (WB) protocols:Immunohistochemistry (IHC) / Immunocytochemistry (ICC) protocols:Chromatin Immunoprecipitation (ChIP) protocols:Dot blot protocols:ELISA protocols:ELISPOT protocols: Flow cytometry / FACS protocols:Immunoprecipitation (IP) protocols: |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [PCK-26] (ab6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [PCK-26] (ab6401)
ab6401 staining pan Cytokeratin in Mouse liver tumor tissue sections by IHC-P (Paraformaldehyde fixed paraffin embedded sections). Tissue was fixed with paraformaldehyde and blocked with maleate buffer blocking solution for 30 minutes and 22ºC. Antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/500) in maleate buffer blocking solution for 16 hours at 22ºC. An undiluted biotin-conjugated Donkey polyclonal to mouse IgG was used as secondary antibody.
This image is courtesy of an Abreview submitted by Dr Asha Seth
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [PCK-26] (ab6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [PCK-26] (ab6401)
ab6401 staining pan Cytokeratin in mouse pancreatic ductal adenocarcinoma (left-hand panel) and mouse pancreatic neoplasia (right-hand panel) sections by immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; heat mediated antigen retrieval was performed. The sample was incubated with primary antibody (1/250) at 4ºC for 8 hours. A Biotin-conjugated Goat polyclonal (1/1000) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
![Western blot - pan Cytokeratin antibody [PCK-26] (ab6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-7.jpg)
Western blot - pan Cytokeratin antibody [PCK-26] (ab6401)
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
All lanes: pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 µg)
Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique
Performed under non-reducing conditions.
Blocking Step: 10% Milk for 1 hour at room temperature
This image is courtesy of an Abreview submitted by Pawel Mazur
![Immunohistochemistry (Frozen sections) - pan Cytokeratin antibody [PCK-26] (ab6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-9.jpg)
Immunohistochemistry (Frozen sections) - pan Cytokeratin antibody [PCK-26] (ab6401)
ab6401 staining pan Cytokeratin in Mouse pancreatic neoplasia tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/250 in PBS) for 8 hours at 4ºC. An Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
![Immunocytochemistry/ Immunofluorescence - pan Cytokeratin antibody [PCK-26] (ab6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-15.jpg)
Immunocytochemistry/ Immunofluorescence - pan Cytokeratin antibody [PCK-26] (ab6401)
ab6401 staining pan Cytokeratin in Human Panc1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone/methanol (1:1) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS) for 2 hours. An Alexa Fluor®488-conjugated DGoat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI.
This image is courtesy of an anonymous Abreview
![pan Cytokeratin antibody [PCK-26] for Western blot in Mouse (6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-5.png)
pan Cytokeratin antibody [PCK-26] for Western blot in Mouse (6401)
![pan Cytokeratin antibody [PCK-26] for IHC-P in Mouse (6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-1.jpg)
pan Cytokeratin antibody [PCK-26] for IHC-P in Mouse (6401)
![pan Cytokeratin antibody [PCK-26] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-3.png)
pan Cytokeratin antibody [PCK-26] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (6401)
![pan Cytokeratin antibody [PCK-26] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-11.png)
pan Cytokeratin antibody [PCK-26] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (6401)
![pan Cytokeratin antibody [PCK-26] for Immunohistochemistry (Frozen sections) in Human (6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-14.jpg)
pan Cytokeratin antibody [PCK-26] for Immunohistochemistry (Frozen sections) in Human (6401)
![pan Cytokeratin antibody [PCK-26] for Western blot in Human (6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-10.png)
pan Cytokeratin antibody [PCK-26] for Western blot in Human (6401)
![pan Cytokeratin antibody [PCK-26] for Immunohistochemistry (Frozen sections) in Mouse (6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-8.png)
pan Cytokeratin antibody [PCK-26] for Immunohistochemistry (Frozen sections) in Mouse (6401)
![pan Cytokeratin antibody [PCK-26] for Immunocytochemistry/ Immunofluorescence in Human (6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-12.png)
pan Cytokeratin antibody [PCK-26] for Immunocytochemistry/ Immunofluorescence in Human (6401)
![pan Cytokeratin antibody [PCK-26] for Immunocytochemistry/ Immunofluorescence in Mouse (6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-13.png)
pan Cytokeratin antibody [PCK-26] for Immunocytochemistry/ Immunofluorescence in Mouse (6401)
![Anti-pan Cytokeratin antibody [PCK-26] for Western blot in Mouse (6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-20.jpg)
Anti-pan Cytokeratin antibody [PCK-26] for Western blot in Mouse (6401)
![pan Cytokeratin antibody [PCK-26] for WB in Mouse (6401)](/ps/Reviews/Images/ab12380_68305.jpg)
pan Cytokeratin antibody [PCK-26] for WB in Mouse (6401)
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