Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
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ab6401 has been referenced in 8 publications.
Publishing research using ab6401? Please let us know so that we can cite the reference in this datasheet
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ab6401 staining pan Cytokeratin in Mouse liver tumor tissue sections by IHC-P (Paraformaldehyde fixed paraffin embedded sections). Tissue was fixed with paraformaldehyde and blocked with maleate buffer blocking solution for 30 minutes and 22ºC. Antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/500) in maleate buffer blocking solution for 16 hours at 22ºC. An undiluted biotin-conjugated Donkey polyclonal to mouse IgG was used as secondary antibody.
This image is courtesy of an Abreview submitted by Dr Asha Seth
ab6401 staining pan Cytokeratin in mouse pancreatic ductal adenocarcinoma (left-hand panel) and mouse pancreatic neoplasia (right-hand panel) sections by immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; heat mediated antigen retrieval was performed. The sample was incubated with primary antibody (1/250) at 4ºC for 8 hours. A Biotin-conjugated Goat polyclonal (1/1000) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
All lanes: pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 µg)
Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique
Performed under non-reducing conditions.
Blocking Step: 10% Milk for 1 hour at room temperature
This image is courtesy of an Abreview submitted by Pawel Mazur
ab6401 staining pan Cytokeratin in Mouse pancreatic neoplasia tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/250 in PBS) for 8 hours at 4ºC. An Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
ab6401 staining pan Cytokeratin in Human Panc1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone/methanol (1:1) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS) for 2 hours. An Alexa Fluor®488-conjugated DGoat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI.
This image is courtesy of an anonymous Abreview
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