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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [PCK-26] (ab6401)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [PCK-26] (ab6401)](/ps/datasheet/images/6/ab6401/pan-Cytokeratin-Primary-antibodies-ab6401-2.jpg)
(enlarge)
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Product Name
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pan Cytokeratin antibody [PCK-26]
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See all pan Cytokeratin antibodies (18)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [PCK-26] to pan Cytokeratin
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Immunogen
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Full length native protein (purified) (Human epidermis).
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Reacts with
(species key)
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Ms, Rat, Many, Chk, Cow, Goat, Rb, Shp
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Specificity
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PCK-26 recognizes the 58 kDa cytokeratin 5, the 56 kDa cytokeratin 6 and the 52 kDa cytokeratin 8 band in immunoblotting (Lane EB et al). It reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues.
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Tested applications
(see key)
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Dot, Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dot: Use at an assay dependent dilution.
Flow Cyt: Use 10µl for 5 x 107 cells (PMID 19855980).
ICC/IF: 1/300.
IF: 1/300.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
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Relevance
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Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors.
There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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PCK-26
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Isotype
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IgG1
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Purity
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Ascites
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Storage buffer
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Ascitic fluid with 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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7.600 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this pan Cytokeratin antibody [PCK-26] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for pan Cytokeratin antibody [PCK-26] images, references, products related to ab6401 and other tools.
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pan Cytokeratin antibody [PCK-26] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan Cytokeratin antibody [PCK-26] (ab6401)
ab6401 staining pan Cytokeratin in Mouse liver tumor tissue sections by IHC-P (Paraformaldehyde fixed paraffin embedded sections). Tissue was fixed with paraformaldehyde and blocked with maleate buffer blocking solution for 30 minutes and 22°C. Antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/500) in maleate buffer blocking solution for 16 hours at 22°C. An undiluted biotin-conjugated Donkey polyclonal to mouse IgG was used as secondary antibody. This image is courtesy of an Abreview submitted by Dr Asha Seth
See Abreview
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References for pan Cytokeratin antibody [PCK-26] (ab6401)
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This product has been used in: (two most recent references)
Kofler K et al. Fluorescence-activated cell sorting of PCK-26 antigen-positive cells enables selection of ovine esophageal epithelial cells with improved viability on scaffolds for esophagus tissue engineering. Pediatr Surg Int 26:97-104 (2010). Flow Cyt, IHC-Fr; Sheep. PubMed: 19855980
Zhang L et al. Adam12 plays a role during uterine decidualization in mice. Cell Tissue Res : (2009). ICC/IF; Mouse. PubMed: 19841944
See all 7 publication references for this product.
If you publish research using ab6401 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to pan Cytokeratin
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pan Cytokeratin antibody [PCK-26] - more information
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Customer reviews (feedback) regarding pan Cytokeratin antibody [PCK-26] |
Customer FAQs regarding pan Cytokeratin antibody [PCK-26] |
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Price and availability of products related to pan Cytokeratin antibody [PCK-26] |
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pan Cytokeratin antibody [PCK-26] - related products:
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pan Cytokeratin antibody [PCK-26] - other tools:
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Contact Abcam with a Technical Enquiry about pan Cytokeratin antibody [PCK-26] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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