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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Synuclein
Overview
Product name
Anti-pan Synuclein antibody
See all pan Synuclein products (5) ...
Description
Rabbit polyclonal to pan Synuclein
Specificity
This antibody recognises all 3 forms of the synucleins.
Tested applications
IHC-P, IHC-Fr, WB, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Chicken, Cow, Xenopus laevis
Immunogen
Synthetic peptide AKEGVVAAAEKTKQGV corresponds to amino acids 11- 26 of the human synuclein protein. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC) and coupled to diphtheria toxoid.
AKEGVVAAAE KTKQGV
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Synuclein
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Immunocytochemistry/ Immunofluorescence-pan Synuclein antibody(ab6176)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab6176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-P: 1/1000IHC-P: 1/1000
IHC-Fr
IHC-Fr: 1/1000IHC-Fr: 1/1000
WB
WB: 1/1000WB: 1/1000
ICC/IF: Use a concentration of 1 µg/ml
Target
Relevance
Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins: alpha, beta and gamma synucleins. Synuclein-alpha is primarily localized at the presynaptic region of axons and it may be involved in the regulation of dopamine release and transport. However, it can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Synuclein-alpha may induce fibrillization of the microtubule-associated protein tau and it reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. Synuclein-alpha is involved in Parkinson's disease, Lewy body dementia, Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome) and it is a major non-amyloid component of the deposits associated with Alzheimer's disease. Synuclein-beta may be a regulator of the SNCA aggregation process. It protects neurons from staurosporine and 6 hydroxy dopamine (6OHDA)-stimulated caspase activation that also involves p53 and contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Gamma synuclein is found primarily in the peripheral nervous system and retina. High levels of expression have been identified in advanced breast carcinomas suggesting a correlation between overexpression of SNCG and breast tumor development.
Cellular localization
Neuronal cytosol and presynaptic terminals
Alternative names
- BCSG1 antibody
- beta synuclein antibody
- Breast cancer specific gene 1 protein antibody
see all
Database links
- Entrez Gene: 281494 Cow
- Entrez Gene: 6620 Human
- Entrez Gene: 6622 Human
- Entrez Gene: 6623 Human
- Entrez Gene: 104069 Mouse
- Entrez Gene: 20617 Mouse
- Entrez Gene: 20618 Mouse
- Entrez Gene: 113893 Rat
see all
Anti-pan Synuclein antibody images:
Immunocytochemistry/ Immunofluorescence-pan Synuclein antibody(ab6176)
ICC/IF image of ab6176 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6176, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-pan Synuclein antibody (ab6176)
This product has been referenced in:
- Näsström Tet al. The lipid peroxidation products 4-oxo-2-nonenal and 4-hydroxy-2-nonenal promote the formation of a-synuclein oligomers with distinct biochemical, morphological, and functional properties. Free Radic Biol Med 50:428-37 (2011). Indirect ELISA; Human.Read more (PubMed: 21130160) »
- Ulusoy Aet al. Co-expression of C-terminal truncated alpha-synuclein enhances full-length alpha-synuclein-induced pathology. Eur J Neurosci 32:409-22 (2010). IHC-P; Rat.Read more (PubMed: 20704592) »
See all 6 publications for this product
Publishing research using ab6176? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"