pan Synuclein antibody (ab6176)

Product Name 

pan Synuclein antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to pan Synuclein

Immunogen 

Synthetic peptide AKEGVVAAAEKTKQGV corresponds to amino acids 11- 26 of the human synuclein protein.
The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC) and coupled to diphtheria toxoid.
BLAST 'AKEGVVAAAEKTKQGV' with or

Reacts with 

Hu, Rat

Predicted to react 
(species key)

Ms, Chk, Cow, Xl
(Due to sequence homology)

Specificity 

This antibody recognises all 3 forms of the synucleins.

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Immunohistochemistry: use at 1/1000
Western Blot: use at 1/1000
Immunoblot: use at 1/1000

Cellular localization 

Neuronal cytosol and presynaptic terminals

Research areas 

Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Synuclein

Relevance 

Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins: alpha, beta and gamma synucleins.
Synuclein-alpha is primarily localized at the presynaptic region of axons and it may be involved in the regulation of dopamine release and transport. However, it can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Synuclein-alpha may induce fibrillization of the microtubule-associated protein tau and it reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. Synuclein-alpha is involved in Parkinson's disease, Lewy body dementia, Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome) and it is a major non-amyloid component of the deposits associated with Alzheimer's disease.
Synuclein-beta may be a regulator of the SNCA aggregation process. It protects neurons from staurosporine and 6 hydroxy dopamine (6OHDA)-stimulated caspase activation that also involves p53 and contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA.
Gamma synuclein is found primarily in the peripheral nervous system and retina. High levels of expression have been identified in advanced breast carcinomas suggesting a correlation between overexpression of SNCG and breast tumor development.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

n/a

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

References for pan Synuclein antibody (ab6176)

  This product has been used in: (two most recent references)

Opazo F et al. Accumulation and clearance of alpha-synuclein aggregates demonstrated by time-lapse imaging. J Neurochem : (2008). PubMed: 18410502

Li W & Lee MK Antiapoptotic property of human alpha-synuclein in neuronal cell lines is associated with the inhibition of caspase-3 but not caspase-9 activity. J Neurochem 93:1542-50 (2005). PubMed: 15935070

See all 3 publication references for this product.


If you publish research using ab6176 please let us know so that we can cite the reference on this datasheet.

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