You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-pan-AKT (phospho T308) antibody (ab38449)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-pan-AKT (phospho T308) antibody
See all pan-AKT products (6) ...

Description

Rabbit polyclonal to pan-AKT (phospho T308)

Specificity

ab38449 recognises endogenous levels of AKT (pT308), AKT2 (pT309) and AKT3 (pT305) only when phosphorylated at those residues.

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 308 (MKTpFC).

Positive control

Human lung carcinoma, extracts from 293 cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - AKT1 (phospho T308) antibody (ab38449)Western blot - AKT1 (phospho T308) antibody (ab38449) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan-AKT (phospho T308) antibody (ab38449)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan-AKT (phospho T308) antibody (ab38449) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab38449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Predi...Read more →

    WB: 1/500 - 1/1000.Predicted molecular weight: 56 kDa.

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Relevance

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphoylate and inactivate several targets, including Bad, Forkhead transcription factors, and caspase 9. Activity of AKT has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to AKT activity, however phosphorylation of T308 has been shown to be absolutely essential for AKT activation.

Cellular localization

Cell Membrane and Cytoplasmic

Alternative names

  • AKT1 antibody
  • AKT2 antibody
  • AKT3 antibody
  • MGC9965 antibody
  • Oncogene AKT1 antibody
  • PKB alpha antibody
  • PKB antibody
  • PKB beta antibody
  • PKBalpha antibody
  • PRKBA antibody
  • PRKBB antibody
  • PRKBG antibody
  • Protein kinase B antibody
  • Protein kinase B beta antibody
  • Protein kinase B gamma antibody
  • RAC alpha antibody
  • RAC antibody
  • RAC gamma antibody
  • RAC PK alpha antibody
  • RAC PK beta antibody
  • RAC PK gamma antibody
  • STK2 antibody
see all

Anti-pan-AKT (phospho T308) antibody images:

  Western blot - AKT1 (phospho T308) antibody (ab38449)

Western blot - AKT1 (phospho T308) antibody (ab38449)

All lanes : Anti-pan-AKT (phospho T308) antibody (ab38449) at 1/500 dilution

Lane 1 : The extracts from 293 cells untreated
Lane 2 : The extracts from 293 cells treated with insulin.

Lysates/proteins at 30 µg per lane.


Predicted band size : 56 kDa
Observed band size : 56 kDa


Lanes can be loaded with 5-30µg of total protein.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan-AKT (phospho T308) antibody (ab38449)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan-AKT (phospho T308) antibody (ab38449)

Immunohistochemical analysis of AKT (phospho T308) expression in paraffin embedded human lung carcinoma tissue, using ab38449 (1/50). Right-hand panel represents a negative control where ab38449 was pre-incubated with the immunizing (blocking) peptide.

References for Anti-pan-AKT (phospho T308) antibody (ab38449)

This product has been referenced in:

  • Hartz AMet al. Estrogen receptor beta signaling through phosphatase and tensin homolog/phosphoinositide 3-kinase/Akt/glycogen synthase kinase 3 down-regulates blood-brain barrier breast cancer resistance protein. J Pharmacol Exp Ther 334:467-76 (2010). WB; Rat.Read more (PubMed: 20460386) »
  • Wagner Met al. Selective expansion of the {beta}-cell compartment in the pancreas of keratinocyte growth factor transgenic mice. Am J Physiol Gastrointest Liver Physiol : (2008).Read more (PubMed: 18372394) »

See all 2 publications for this product

Publishing research using ab38449? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"