Overview

  • Product nameAnti-pan Cadherin antibodySee all pan Cadherin primary antibodies ...
  • Description
    Rabbit polyclonal to pan Cadherin
  • SpecificityRecognises all cadherin members. Rabbit Anti-Pan Cadherin shows specific reactivity with a distinct 135 kD band on chicken or rabbit heart extract blots using indirect immunoblotting.
  • Tested applicationsIHC-P, WB, ICC/IF, Electron Microscopy, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Xenopus laevis, Zebrafish
    Predicted to work with: Rabbit, Bird, Fish, Amphibians
  • Immunogen

    Synthetic peptide: DYDYLNDWGPRFKKLADMYGGGDD conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 889-912 of Chicken pan Cadherin.

  • General notesImmunohistochemistry This antibody has not been tested on frozen sections but has been tested on both cultured cells and formalin-fixed, paraffin-embedded, protease digested sections so chances are it will work on frozen sections. It has also been tested on heart sections and MDBK cells. This antibody should work on bovine samples although we have not tested it specifically.


    This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

Properties

Applications

Our Abpromise guarantee covers the use of ab6529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/1000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB 1/200. On chicken and rabbit heart extract this antibody detects a single band at 135 kD.
ICC/IF 1/100. Fix cells with 2-4% paraformaldehyde for 10 minutes at room temperature, followed by 10-minute incubation with 0.2% Triton X-100 (see Gellersen et al 2007 and Kaur et al 2006).
Electron Microscopy Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution. Fix with ice cold ethanol.

Target

Anti-pan Cadherin antibody images

  • Immunofluorescent imaging of human cells (U2OS) with ab6529 confirms the specificity of this antibody.  Pan cadherin antibody recognises predominantly membranous signal with some fainter cytoplasmic staining corresponding to soluble cadherin. No nuclear staining is seen. 

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees C. Nuclei were stained with Hoechst stain.

  • ab6529 staining pan Cadherin in human bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed and blocked in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted, 1/200 and incubated with sample for 9 hour at 4°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/200 was used as secondary.

  • This image is courtesy of an anonymous Abreview

    See Abreview

  • ab6529 staining pan Cadherin in rat kidney tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 2% BSA for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/200 dilution, for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

    See Abreview

  • ab6529 staining pan Cadherin in murine liver cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.4% Triton X-100 and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 17 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) used at a 1/1000 dilution. The other secondary antibody used was an Alexa Fluor® 488 (green) goat anti-mouse IgG for detection of a specific cytosolic protein.

    See Abreview

References for Anti-pan Cadherin antibody (ab6529)

This product has been referenced in:
  • Buensuceso AV & Deroo BJ The ephrin signaling pathway regulates morphology and adhesion of mouse granulosa cells in vitro. Biol Reprod 88:25 (2013). IHC-Fr ; Mouse . Read more (PubMed: 23242526) »
  • Mondini A  et al. Hypertension-linked mutation of a-adducin increases CFTR surface expression and activity in HEK and cultured rat distal convoluted tubule cells. PLoS One 7:e52014 (2012). WB ; Rat . Read more (PubMed: 23284854) »

See all 12 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Roti­-ImmunoBlock as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 26°C
Sample Cat Cell (CrFK)
Specification CrFK
Permeabilization Yes - Triton X-100 0.2 %
Fixative Paraformaldehyde
Username

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Submitted Jan 27 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Liver (20 um section))
Specification Liver (20 um section)
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Username

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Verified customer

Submitted Jan 18 2013

Merci de nous avoir contactés.

Comme convenu, j'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Selon nos archives, vous avez désormais deux avoirs &...

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Merci de nous avoir contactés.

Je suis désolée de n'avoir pu améliorer vos résultats.

Je vous propose un remboursement ou un avoir. Sinon je peux également vous proposer un remplacement ave...

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Merci d'avoir pris le temps de nous envoyer votre protocole et images. Je suis désolée d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab6529

Notre politique est d'offrir tout ...

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Je suis désolée d'apprendre que ab6529 ne vous donne pas de bons résultats en IF.

Veuillez trouver ci-joint un questionnaire qui nous permet de regrouper le plus d'informations possible sur le protocole que vous avez uti...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepG2)
Specification HepG2
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 29 2012

Thank you for contacting us.
Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.
We are happy to reassure our customers that all of our products are covered by our Abpromise, which guara...

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Thank you for the images of the staining with ab22744.

I agree the staining is not what is usually observed for cadherin. I think you are expecting something like what one of our reviewers saw. The data is at this link:

http://www...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab22744.

To check the status of the order please contact o...

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