The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Use at a concentration of 2.5 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.