Anti-pan Cadherin antibody [mAbcam22744] (ab22744)
Key features and details
- Mouse monoclonal [mAbcam22744] to pan Cadherin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-pan Cadherin antibody [mAbcam22744]
See all pan Cadherin primary antibodies -
Description
Mouse monoclonal [mAbcam22744] to pan Cadherin -
Host species
Mouse -
Specificity
Detects a weaker band in human heart than in rat heart. -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Dog, Xenopus laevis, Monkey, Zebrafish, African green monkey -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
IgG fraction -
Primary antibody notes
This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type. -
Clonality
Monoclonal -
Clone number
mAbcam22744 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (4) |
Use a concentration of 5 µg/ml.
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WB | (6) |
1/1000. Detects a band of approximately 125-140 kDa (predicted molecular weight: 125 kDa).
Abcam recommends using 3-5% milk as the blocking agent. Please see Western Blot data below. |
Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/1000. Detects a band of approximately 125-140 kDa (predicted molecular weight: 125 kDa). Abcam recommends using 3-5% milk as the blocking agent. Please see Western Blot data below. |
Target
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Relevance
Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis. -
Database links
- Entrez Gene: 1001 Human
- Entrez Gene: 1002 Human
- Entrez Gene: 1003 Human
- Entrez Gene: 1004 Human
- Entrez Gene: 1005 Human
- Entrez Gene: 1006 Human
- Entrez Gene: 1008 Human
- Entrez Gene: 1009 Human
see all -
Alternative names
- Cadherin antibody
- CDH3 antibody
- CDHP antibody
see all
Images
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Lane 1 : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml (Blocked in 5% BSA)
Lane 2 : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml (Blocked in 5% Milk)
All lanes : Heart (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 125 kDa
Exposure time: 30 seconds -
ICC/IF image of ab22744 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22744, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
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All lanes : Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1 µg/ml
Lane 1 : Heart (Rat) Tissue Lysate (blocked with 5% Milk)
Lane 2 : Heart (Mouse) Tissue Lysate (blocked with 5% Milk)
Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue (blocked with 5% Milk)
Lane 4 : Heart (Rat) Tissue Lysate (blocked with 3% Milk)
Lane 5 : Heart (Mouse) Tissue Lysate (blocked with 3% Milk)
Lane 6 : Heart (Human) Tissue Lysate - adult normal tissue (blocked with 3% Milk)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 125 kDa
Observed band size: 125 kDa
Additional bands at: 25 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
ab22744 staining pan Cadherin in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol, permeabilised in 0.5% (w/v) saponin and then blocked using 10% serum for 2 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 2 hours at 23°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. Counterstained with DAPI (blue).
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Anti-pan Cadherin antibody [mAbcam22744] (ab22744) at 1/500 dilution + Mouse cultured cortical neurons at 20 µg
Secondary
HRP-conjugated Goat Anti-Mouse IgG (H+L) polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 125 kDa
Exposure time: 1 minute
Blocking performed with 5% milk for 1 hour.
Primary diluted with PBS + 0.5% Tween20 and incubated for 12 hours at 4°C
Performed under denaturing conditions. -
ICC/IF image of ab22744 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22744, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab22744 has been referenced in 21 publications.
- Lohoff T et al. Integration of spatial and single-cell transcriptomic data elucidates mouse organogenesis. Nat Biotechnol 40:74-85 (2022). PubMed: 34489600
- Peischard S et al. Virus-induced inhibition of cardiac pacemaker channel HCN4 triggers bradycardia in human-induced stem cell system. Cell Mol Life Sci 79:440 (2022). PubMed: 35864219
- Geng B et al. Near-infrared phosphorescent carbon dots for sonodynamic precision tumor therapy. Nat Commun 13:5735 (2022). PubMed: 36175446
- Kong LR et al. A common MET polymorphism harnesses HER2 signaling to drive aggressive squamous cell carcinoma. Nat Commun 11:1556 (2020). PubMed: 32214092
- Joksimovic SL et al. Selective inhibition of CaV3.2 channels reverses hyperexcitability of peripheral nociceptors and alleviates postsurgical pain. Sci Signal 11:N/A (2018). PubMed: 30154101