Overview

  • Product namepan Caspase (active) Red Staining Kit
    See all Caspase kits
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's pan Caspase (active) Red Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes the caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent in situ marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of caspases plays a central role in apoptosis.

  • Tested applicationsSuitable for: FM, Flow Cyt, Functional Studiesmore details

Properties

  • RelevanceCaspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • Cellular localizationCytoplasmic

Applications

Our Abpromise guarantee covers the use of ab65616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
Functional Studies Use at an assay dependent dilution.

Protocols

References for pan Caspase (active) Red Staining Kit (ab65616)

ab65616 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab65616.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"