Overview

  • Product nameAnti-pan CEACAM antibody [TET2]
    See all pan CEACAM primary antibodies
  • Description
    Mouse monoclonal [TET2] to pan CEACAM
  • Specificityab26283 is a broadly reactive antibody which reacts with most of the CEACAM molecules characterized to date (except CEACAM3, CEACAM4 and CEACAM7)4. It can be used to detect CEACAM1 (BGP/CD66a), CEACAM5 (CEA/CD66e), CEACAM6 (NCA/CD66c) and CEACAM8 (CGM6/CD66b) on transiently transfected BOSC23 cells by flow cytometry.
  • Tested applicationsSuitable for: Flow Cyt, ELISA, WB, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    ab26283 was generated by immunisation of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberTET2
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 21645414NK cells were isolated from whole PBMC by negative magnetic bead fractionation.

Target

  • RelevanceCEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • Antigen CD66 antibody
    • Biliary glycoprotein antibody
    • Carcinoembryonic antigen related cell adhesion molecule 1 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 3 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 4 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 5 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 6 antibody
    • CD66a antigen antibody
    • CD66D antibody
    • CD66e antibody
    • CEACAM1 antibody
    • CEACAM3 antibody
    • CEACAM4 antibody
    • CEACAM5 antibody
    • CEACAM6 antibody
    see all

Anti-pan CEACAM antibody [TET2] images

  • Overlay histogram showing Caco 2 cells stained with ab26283 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26283, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-pan CEACAM antibody [TET2] (ab26283)

This product has been referenced in:
  • Moreland AJ  et al. Characterization of killer immunoglobulin-like receptor genetics and comprehensive genotyping by pyrosequencing in rhesus macaques. BMC Genomics 12:295 (2011). IP ; Rhesus monkey . Read more (PubMed: 21645414) »
  • Graham SA  et al. Identification of Neutrophil Granule Glycoproteins as Lewisx-containing Ligands Cleared by the Scavenger Receptor C-type Lectin. J Biol Chem 286:24336-49 (2011). WB . Read more (PubMed: 21561871) »

See all 2 Publications for this product

Product Wall

Merci de nous avoir contactés.

L'anti-pan CEACAM [TET2] (ab26283) est un anticorps purifié sur protéine G et n'est pas conjugué à un fluorophore ou à une enzyme.

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Thank you for your inquiry. I was liaising with the laboratories to find out more about our pan-CEACAM- and CEACAM1-antibodies. Unfortunately, it turned out that we do not have any suitable antibody available: The immunogens of our CEACAM1 anti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"