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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins

Anti-pan Cytokeratin antibody [5D3 + LP34] (ab17153)

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Publishing research using ab17153? Please let us know so that we can cite the reference in this datasheet

ab17153 has not yet been referenced specifically in any publications.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Immunocytochemistry/ Immunofluorescence-pan Cytokeratin antibody [5D3 + LP34](ab17153)

ICC/IF image of ab17153 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17153, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - pan Cytokeratin antibody [5D3 + LP34] (ab17153)

Anti-pan Cytokeratin antibody [5D3 + LP34] (ab17153) at 1/500 dilution ((in 5% Marvel TBS-T). Incubation for 1 hour at 22°C) + Whole cell lysate of human HT1080 cells at 25 µg

Secondary
An HRP-conjugated Sheep anti-mouse IgG monoclonal at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Observed band size : 45 kDa (why is the actual band size different from the predicted?)

Exposure time : 15 minutes

Blocking Step: 5% Milk for 1 hour at 22°C

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [5D3 + LP34] (ab17153)

ab17153 staining human skin and lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab17153 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Carl Hobbs

Anti-pan Cytokeratin antibody [5D3 + LP34] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (17153)

pan Cytokeratin antibody [5D3 + LP34] for Western blot in Mouse (17153)

pan Cytokeratin antibody [5D3 + LP34] for Western blot in Human (17153)

Anti-pan Cytokeratin antibody [5D3 + LP34] for Immunocytochemistry/ Immunofluorescence in Human (17153)

ab27230	Goat polyclonal Secondary Antibody to Mouse IgG - heavy chain (10nm Gold), pre-adsorbed (ab27230)
ab27233	Goat polyclonal Secondary Antibody to Mouse IgG - heavy chain (5nm Gold), pre-adsorbed (ab27233)
ab27241	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (10nm Gold), pre-adsorbed (ab27241)
ab27242	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (20nm Gold), pre-adsorbed (ab27242)
ab27244	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (5nm Gold), pre-adsorbed (ab27244)
ab60313	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 488) (ab60313)
ab60316	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 546) (ab60316)
ab60318	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642) (ab60318)
ab96871	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488) (ab96871)
ab96873	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 594) (ab96873)
ab96875	Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488) (ab96875)
ab96877	Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 594) (ab96877)
ab97015	Goat polyclonal Secondary Antibody to Mouse IgG - Fc (DyLight® 488) (ab97015)
ab97017	Goat polyclonal Secondary Antibody to Mouse IgG - Fc (DyLight® 594) (ab97017)
ab97019	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (ab97019)
ab97020	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020)
ab97021	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab97021)
ab97022	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab97022)
ab97023	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023)
ab97024	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (PE) (ab97024)
ab97025	Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Agarose) (ab97025)
ab97026	Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (ab97026)
ab97027	Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97027)
ab97028	Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab97028)
ab97029	Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab97029)
ab97030	Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97030)
ab97042	Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (ab97042)
ab97043	Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043)
ab97044	Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab97044)
ab97045	Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab97045)
ab97046	Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046)
ab97261	Goat polyclonal Secondary Antibody to Mouse IgG - Fc (ab97261)
ab97262	Goat polyclonal Secondary Antibody to Mouse IgG - Fc (AP) (ab97262)
ab97263	Goat polyclonal Secondary Antibody to Mouse IgG - Fc (Biotin) (ab97263)
ab97264	Goat polyclonal Secondary Antibody to Mouse IgG - Fc (FITC) (ab97264)
ab97265	Goat polyclonal Secondary Antibody to Mouse IgG - Fc (HRP) (ab97265)
ab102454	Sheep polyclonal Secondary Antibody to Mouse IgG - H&L (ab102454)
ab102455	Sheep polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab102455)
ab102456	Sheep polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab102456)
ab102457	Sheep polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab102457)
ab102458	Sheep polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab102458)

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Anti-pan Cytokeratin antibody [5D3 + LP34] (ab17153)

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Immunocytochemistry/ Immunofluorescence-pan Cytokeratin antibody [5D3 + LP34](ab17153)

Western blot - pan Cytokeratin antibody [5D3 + LP34] (ab17153)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [5D3 + LP34] (ab17153)

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