Anti-pan Cytokeratin antibody [5D3 & LP34], prediluted (ab848)

Overview

  • Product nameAnti-pan Cytokeratin antibody [5D3 & LP34], prediluted
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [5D3 & LP34] to pan Cytokeratin, prediluted
  • SpecificityThis antibody is a cocktail of two different clones; 5D3 and LP53. This antibody stains cytokeratin 5,6,8 and 18. This antibody has been designed for a broad spectrum screening of cytokeratins. It stains carcinomas of simple and squamous origin.
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cytokeratins from human breast carcinoma cell line MCF-7 and detergent insoluble fraction of psoriatic epidermis.

  • General notesIsotype IgG2a and IgG1

Properties

Applications

Our Abpromise guarantee covers the use of ab848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
  • Application notesThis antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
    Proteolytic digestion of tissue sections is required prior to immunostaining.
  • Target

    • RelevanceCytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
    • Cellular localizationCytoplasmic
    • Database links

    References for Anti-pan Cytokeratin antibody [5D3 & LP34], prediluted (ab848)

    ab848 has not yet been referenced specifically in any publications.

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