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Human epidermal keratin
Our Abpromise guarantee covers the use of ab80826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boiling tissue sections in 1mM EDTA (pH 8.0), for 10-20min followed by cooling at RT for 20min is required.|
Immunohistochemical analysis of rat bladder tissue, labeling pan Cytokeratin with ab80826. Samples were formaldehyde fixed, heat mediated antigen retrieval was perfmormed with 10mM citrate buffer, and blocking was with 10% serum for 2 hours at 23°C. Incubation with ab80826 (diluted 1/1000) was for 17 hours at 4°C.