Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)


  • Product nameAnti-pan Cytokeratin antibody [AE1+AE3], prediluted
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [AE1+AE3] to pan Cytokeratin, prediluted
  • SpecificityThis antibody mixture recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52 kDa.
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Dog, Human, Fish, Zebrafish
  • Immunogen

    Human epidermal cytokeratins

  • Positive control
    • Skin or adenocarcinoma.



Our Abpromise guarantee covers the use of ab961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19852942
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.


  • RelevanceCytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localizationCytoplasmic
  • Database links

Anti-pan Cytokeratin antibody [AE1+AE3], prediluted images

  • ab961 at a 1/100 dilution staining pan Cytokeratin in human urothelium-like cells in culture by Immunocytochemistry/ Immunofluorescence. Counterstained with DAPI.
  • Immunohistochemical analysis of zebrafish pancreatic tissue, staining pan Cytokeratin with ab961, at 1/500 dilution.

References for Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961)

This product has been referenced in:
  • Leibowitz BJ  et al. Ionizing irradiation induces acute haematopoietic syndrome and gastrointestinal syndrome independently in mice. Nat Commun 5:3494 (2014). Mouse . Read more (PubMed: 24637717) »
  • Igawa S  et al. Circulating tumor cells as a prognostic factor in patients with small cell lung cancer. Oncol Lett 7:1469-1473 (2014). ICC/IF ; Human . Read more (PubMed: 24765158) »

See all 13 Publications for this product

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I can confirm that the Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961) can be used for applications without further dilutions.

However, every experiment is different and may need optimisation b...

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Merci de nous avoir contactés.

L'anticorps prédilué ab82612 (Anti-Cytokeratin [MNF116]) a une concentration entre 1 et 3 µg/ml.
L'anticorps ab28028 (Anti-Vimentin [Vim 3B4]) est un supernageant, la concentration ...

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Thank you for contacting us.

The antibodies ab6401 and ab11214 are from the same clone [PCK-26], so they are the same antibody with similar binding characteristics. The difference is that ab6401 is not purified and is not conjugated while ab11...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Dog-Kidney)
Specification Dog-Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rm°C

Jing Ma

Verified customer

Submitted Aug 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (tumor)
Specification tumor
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 01 2009

Thank you for your enquiry and patience. I was able to obtain the following information from the originators of these antibodies: For ab11213, "this product should recognise cytokeratins 1, 4, 5, 6, 8,10, 13, 18 and 19. Based on literature, respirat...

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Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

According to the datasheet, this antibody recognizes mouse protein. However, we do not have data on mouse kidney tissue particularly. Please have a look at the published references, you may find some more information.