Anti-pan Cytokeratin antibody [B311.1] (ab8474)

Overview

  • Product nameAnti-pan Cytokeratin antibody [B311.1]
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [B311.1] to pan Cytokeratin
  • SpecificityThis antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond to cytokeratins 4,5,6,8,10,13 and 18.
  • Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Other Immunogen Type corresponding to Human pan Cytokeratin. Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.

  • Positive control
    • Skin, Colonic Carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab8474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP 1/2000.
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Prolonged fixation in buffered formalin can destroy the epitope.
WB 1/100 - 1/400. Detects a band of approximately 49-59 kDa.

Target

  • RelevanceCytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localizationCytoplasmic
  • Database links

References for Anti-pan Cytokeratin antibody [B311.1] (ab8474)

ab8474 has not yet been referenced specifically in any publications.

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