Anti-pan Cytokeratin antibody [C-11] (ab118817)

Overview

  • Product nameAnti-pan Cytokeratin antibody [C-11]
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [C-11] to pan Cytokeratin
  • SpecificityReacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all Mammals
  • Immunogen

    Tissue, cells or virus corresponding to Human pan Cytokeratin.

  • Positive control
    • Human Breast tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab118817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceCytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localizationCytoplasmic
  • Database links

Anti-pan Cytokeratin antibody [C-11] images

  • ab118817, at 10 µg/ml, staining Keratin in formalin-fixed, paraffin-embedded Human Breast tissue by Immunohistochemistry using a biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-pan Cytokeratin antibody [C-11] (ab118817)

ab118817 has not yet been referenced specifically in any publications.

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