Anti-pan Cytokeratin antibody [C-11] (ab7753)

Overview

  • Product name
    Anti-pan Cytokeratin antibody [C-11]
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [C-11] to pan Cytokeratin
  • Specificity
    Cytokeratin peptides 4,5,6,8,10,13,18.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Goat, Cow, Human, Pig, Common marmoset, Bat
    Predicted to work with: a wide range of other species, Mammal
  • Immunogen

    A keratin-enriched preparation from human epidermoid carcinoma cell line A431.

  • Positive control
    • ICC/IF: A549 and mouse hepatocytes (see reviews). WB: A431 cell lysate. Flow Cyt: HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    Purified from ascites using precipitation methods.
  • Clonality
    Monoclonal
  • Clone number
    C-11
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500. Fix with 4% PFA and permeabilize with 0.5 % Triton (See Abreview).
Flow Cyt Use a concentration of 0.5 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. 1/500 - 1/1000 (See Abreview).
IP Use at an assay dependent concentration.

Target

  • Relevance
    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localization
    Cytoplasmic
  • Database links

Anti-pan Cytokeratin antibody [C-11] images

  • ab7753 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab7753 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • ab7753 staining pan Cytokeratin in Rat urinary bladder tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C. Samples were incubated with primary antibody (1/150 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab7753 staining pan Cytokeration in the Human NSCLC cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in 0.3% Triton, 5% Goat Serum in PBS) for 1 hour. An Alexa Fluor® 555-conjugated Goat polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-pan Cytokeratin antibody [C-11] (ab7753) + human A431 cell line on 10% gel.This is a pan cytokeratin antibody and there are several cytokeratin bands between about 40 and 55 kDa. The identity of the particular cytokeratin that each band corresponds to has not been determined.
  • Overlay histogram showing HeLa cells stained with ab7753 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7753, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab7753 staining rat embryonic skin/organ sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab77539 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • ab7753 staining pan Cytokeratin in Pig urether tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/500) for 3 hours at 20°C. A Cy2®-conjugated Goat anti-mouse monoclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab7753 staining pan Cytokeration in Cow Airway Epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% serum for 15 minutes at 37°C. Samples were incubated with primary antibody (1/500 in PBS/1%BSA) for 1 hour at 37°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/50) was used as the secondary antibody.

    See Abreview

  • ab7753 staining pan Cytokeratin in Mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/250 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab7753 staining pan Cytoketatin in mouse hepatocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 0.5% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. A Cy3®-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab7753 staining pan Cytokeratin in guinae pig breast carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

References for Anti-pan Cytokeratin antibody [C-11] (ab7753)

This product has been referenced in:
  • Shivakumar P  et al. Preferential TNFa signaling via TNFR2 regulates epithelial injury and duct obstruction in experimental biliary atresia. JCI Insight 2:e88747 (2017). Read more (PubMed: 28289704) »
  • Balusu S  et al. Identification of a novel mechanism of blood-brain communication during peripheral inflammation via choroid plexus-derived extracellular vesicles. EMBO Mol Med 8:1162-1183 (2016). Read more (PubMed: 27596437) »

See all 24 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - other (A549)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
A549
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jun 06 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human primary airway epithelial and fibroblast cel)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
10 µg
Specification
Human primary airway epithelial and fibroblast cel
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Aaron Gardner

Verified customer

Submitted Oct 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Primary pulmonary epithelial cells cultured at air)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate
Permeabilization
No
Specification
Primary pulmonary epithelial cells cultured at air
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Dr. Aaron Gardner

Verified customer

Submitted Oct 13 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HuCCA-1 (cholangiocarcinoma))
Permeabilization
Yes - 0.5% Tween-20 in PBS
Specification
HuCCA-1 (cholangiocarcinoma)
Blocking step
Blocking Buffer (ab126587) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
95% ethanol+acetic acid
Username

Sittisak

Verified customer

Submitted Aug 11 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 23°C
Sample
Mouse Cell (hepatocytes)
Specification
hepatocytes
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 20 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Pig Tissue sections (Small intestine)
Specification
Small intestine
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 20 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human NSCLC Line)
Specification
Human NSCLC Line
Permeabilization
Yes - 0.3% Triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 06 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Cow Cell (Airway Epithelial Cell)
Specification
Airway Epithelial Cell
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Formaldehyde
Username

E Grahame

Verified customer

Submitted Feb 18 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Rat Tissue sections (Urinary bladder)
Specification
Urinary bladder
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 13 2014

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Endometriotic epithelial)
Specification
Endometriotic epithelial
Permeabilization
Yes - 2 % Triton x-100
Fixative
Paraformaldehyde
Username

Dr. Brett Mckinnon

Verified customer

Submitted Jun 10 2013

1-10 of 25 Abreviews or Q&A

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