Anti-pan Cytokeratin antibody [C-11] (Allophycocyanin) (ab106166)

Overview

  • Product nameAnti-pan Cytokeratin antibody [C-11] (Allophycocyanin)
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [C-11] to pan Cytokeratin (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Specificityab106166 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity

    Predicted to work with: all Mammals
  • Immunogen

    Keratin-enriched preparation from Human epidermoid carcinoma cell line A431.

Properties

Applications

Our Abpromise guarantee covers the use of ab106166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 5 µg/ml. ab37391-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceCytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localizationCytoplasmic

References for Anti-pan Cytokeratin antibody [C-11] (Allophycocyanin) (ab106166)

This product has been referenced in:
  • Turner ML  et al. Epithelial and stromal cells of bovine endometrium have roles in innate immunity and initiate inflammatory responses to bacterial lipopeptides in vitro via Toll-like receptors TLR2, TLR1, and TLR6. Endocrinology 155:1453-65 (2014). Flow Cyt ; Cow . Read more (PubMed: 24437488) »
  • Hamakawa H  et al. Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis. Res Commun Mol Pathol Pharmacol 101:115-26 (1998). Read more (PubMed: 9821208) »

See all 3 Publications for this product

Product Wall

;anticorps ab106166 est à €370 et a une concentration de 0.1mg/ml. Lot 1408490 est en stock en Grande Bretagne. Le control isotype pour cet anticorps est ab91358 à €350. Il est à une concentration de 0.025 mg/ml et est en...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"