APC Anti-pan Cytokeratin antibody [C-11] (ab106166)
Key features and details
- APC Mouse monoclonal [C-11] to pan Cytokeratin
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
APC Anti-pan Cytokeratin antibody [C-11]
See all pan Cytokeratin primary antibodies -
Description
APC Mouse monoclonal [C-11] to pan Cytokeratin -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Specificity
ab106166 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mammals -
Immunogen
Tissue, cells or virus corresponding to Human pan Cytokeratin.
-
Positive control
- Flow Cyt: HeLa cells
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.098% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading...
-
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
C-11 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use a concentration of 1 µg/ml.
|
Notes |
---|
Flow Cyt (Intra)
Use a concentration of 1 µg/ml. |
Target
-
Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
-
Alternative names
- pan ck antibody
- pan-ck antibody
- panck antibody
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab106166 has been referenced in 4 publications.
- Cronin JG et al. Signal transducer and activator of transcription-3 licenses Toll-like receptor 4-dependent interleukin (IL)-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells. Mucosal Immunol 9:1125-36 (2016). Flow Cyt ; Cow . PubMed: 26813342
- Turner ML et al. Epithelial and stromal cells of bovine endometrium have roles in innate immunity and initiate inflammatory responses to bacterial lipopeptides in vitro via Toll-like receptors TLR2, TLR1, and TLR6. Endocrinology 155:1453-65 (2014). Flow Cyt ; Cow . PubMed: 24437488
- Hamakawa H et al. Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis. Res Commun Mol Pathol Pharmacol 101:115-26 (1998). PubMed: 9821208
- Bártek J et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol 164:215-24 (1991). PubMed: 1716305