Anti-pan Cytokeratin antibody [PCK-26] (ab6401)

Overview

  • Product nameAnti-pan Cytokeratin antibody [PCK-26]
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [PCK-26] to pan Cytokeratin
  • Tested applicationsSuitable for: WB, IHC-FoFr, IHC-P, IHC-Fr, Dot blot, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Sheep, Human, Pig
    Predicted to work with: Rat, Rabbit, Goat, Chicken, Cow, a wide range of other species
  • Immunogen

    Full length native protein (purified) (Human epidermis).

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR210999, GR88954. Lot numbers other than GR210999, GR88954 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

Applications

Our Abpromise guarantee covers the use of ab6401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 19855980

Use at an assay dependent concentration.

Use 10µl for 5 x 107 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/300.

Target

  • RelevanceCytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localizationCytoplasmic
  • Database links

Anti-pan Cytokeratin antibody [PCK-26] images

  • ab6401 staining pan Cytokeratin in Mouse liver tumor tissue sections by IHC-P (Paraformaldehyde fixed paraffin embedded sections). Tissue was fixed with paraformaldehyde and blocked with maleate buffer blocking solution for 30 minutes and 22°C. Antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/500) in maleate buffer blocking solution for 16 hours at 22°C. An undiluted biotin-conjugated Donkey polyclonal to mouse IgG was used as secondary antibody.

    See Abreview

  • ab6401 staining pan Cytokeratin in mouse pancreatic ductal adenocarcinoma (left-hand panel) and mouse pancreatic neoplasia (right-hand panel) sections by immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; heat mediated antigen retrieval was performed. The sample was incubated with primary antibody (1/250) at 4°C for 8 hours. A Biotin-conjugated Goat polyclonal (1/1000) was used as the secondary antibody.

    See Abreview



  • Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Pawel Mazur

    All lanes: pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 µg)

    Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique

    Performed under non-reducing conditions.

    Blocking Step: 10% Milk for 1 hour at room temperature

    See Abreview

  • ab6401 staining pan Cytokeratin in Mouse pancreatic neoplasia tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/250 in PBS) for 8 hours at 4°C. An Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab6401 staining pan Cytokeratin in Human Panc1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone/methanol (1:1) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS) for 2 hours. An Alexa Fluor®488-conjugated DGoat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI.

    See Abreview

References for Anti-pan Cytokeratin antibody [PCK-26] (ab6401)

This product has been referenced in:
  • Liu W  et al. Hepatic epithelioid angiomyolipoma is a rare and potentially severe but treatable tumor: A report of three cases and review of the literature. Oncol Lett 11:3669-3675 (2016). IHC ; Human . Read more (PubMed: 27313680) »
  • Figueiredo M  et al. Notch and Hedgehog in the thymus/parathyroid common primordium: Crosstalk in organ formation. Dev Biol 418:268-82 (2016). Read more (PubMed: 27544844) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Normal kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization No
Specification Normal kidney
Blocking step (agent) for 15 minute(s) · Concentration: 3% · Temperature: 27°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 24 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
Sample Pig Tissue sections (Skin)
Specification Skin
Permeabilization Yes - 0.05% Tween 20
Fixative 10% Formalin
Username

Mr. jesse wu

Verified customer

Submitted Oct 30 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
Sample Human Tissue sections (Skin)
Specification Skin
Permeabilization Yes - 0.05% Tween 20
Fixative 10% Formalin
Username

Abcam user community

Verified customer

Submitted Oct 29 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
Sample Rabbit Tissue sections (Skin)
Specification Skin
Permeabilization Yes - 0.05% Tween 20
Fixative 10% Formalin
Username

Abcam user community

Verified customer

Submitted Oct 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse lung)
Specification Mouse lung
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization Yes - Trion X-100
Blocking step 2% horse serum plus mouse Fab IgG fragment as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Mar 28 2013

Thank you for contacting us.
Attached are the two Certificates of Compliance you have requested.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products...

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The antibodies ab6401 and ab11214 are from the same clone [PCK-26], so they are the same antibody with similar binding characteristics. The difference is that ab6401 is not purified and is not conjugated while ab11...

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I have found some references which support that the pan Cytokeratin and E Cadherin would be expressed by epidermal keratinocytes:

http://jmm.sgmjournals.org/content/56/7/971.full

http://www.uni...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (multiple tissues)
Loading amount 40 µg
Specification multiple tissues
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Kris Frese

Verified customer

Submitted Jan 06 2012

Thank you for contacting us. The background should be black. I am sorry we never had a question like this before. It looks like this problem might be due to instrumentation then antibodies itself. I have following recommendations that might hel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"