Anti-pan Cytokeratin antibody [PCK-26] (ab6401)

Overview

  • Product name
    Anti-pan Cytokeratin antibody [PCK-26]
    See all pan Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [PCK-26] to pan Cytokeratin
  • Tested applications
    Suitable for: WB, IHC-FoFr, IHC-P, IHC-Fr, Dot blot, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Sheep, Human, Pig
    Predicted to work with: Rat, Rabbit, Goat, Chicken, Cow, a wide range of other species
  • Immunogen

    Full length native protein (purified) corresponding to Human pan Cytokeratin.

  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Proprietary Purification
  • Purification notes
    Purified from Ascites
  • Clonality
    Monoclonal
  • Clone number
    PCK-26
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 19855980

Use at an assay dependent concentration.

Use 10µl for 5 x 107 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/300.

Target

  • Relevance
    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • Cellular localization
    Cytoplasmic
  • Database links

Anti-pan Cytokeratin antibody [PCK-26] images

  • ab6401 staining pan Cytokeratin in Mouse liver tumor tissue sections by IHC-P (Paraformaldehyde fixed paraffin embedded sections). Tissue was fixed with paraformaldehyde and blocked with maleate buffer blocking solution for 30 minutes and 22°C. Antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/500) in maleate buffer blocking solution for 16 hours at 22°C. An undiluted biotin-conjugated Donkey polyclonal to mouse IgG was used as secondary antibody.

    See Abreview

  • Lane 1 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 2.5 µg/ml
    Lane 2 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 5 µg/ml
    Lane 3 : Anti-pan Cytokeratin antibody [PCK-26] (ab6401) at 10 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HeLa cell lysate

    Secondary
    Peroxidase-conjugated goat anti-mouse IgG
  • ab6401 staining pan Cytokeratin in Human Panc1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone/methanol (1:1) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS) for 2 hours. An Alexa Fluor®488-conjugated DGoat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI.

    See Abreview



  • Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Pawel Mazur

    All lanes: pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 µg)

    Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique

    Performed under non-reducing conditions.

    Blocking Step: 10% Milk for 1 hour at room temperature

    See Abreview

  • ab6401 staining pan Cytokeratin in mouse pancreatic ductal adenocarcinoma (left-hand panel) and mouse pancreatic neoplasia (right-hand panel) sections by immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; heat mediated antigen retrieval was performed. The sample was incubated with primary antibody (1/250) at 4°C for 8 hours. A Biotin-conjugated Goat polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab6401 staining pan Cytokeratin in Mouse pancreatic neoplasia tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/250 in PBS) for 8 hours at 4°C. An Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References for Anti-pan Cytokeratin antibody [PCK-26] (ab6401)

This product has been referenced in:
  • He Z  et al. Epithelial-derived IL-33 promotes intestinal tumorigenesis in Apc (Min/+) mice. Sci Rep 7:5520 (2017). Flow Cyt . Read more (PubMed: 28710436) »
  • Liu W  et al. Hepatic epithelioid angiomyolipoma is a rare and potentially severe but treatable tumor: A report of three cases and review of the literature. Oncol Lett 11:3669-3675 (2016). IHC ; Human . Read more (PubMed: 27313680) »

See all 21 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Normal kidney
Blocking step
(agent) for 15 minute(s) · Concentration: 3% · Temperature: 27°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 24 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
Sample
Pig Tissue sections (Skin)
Specification
Skin
Permeabilization
Yes - 0.05% Tween 20
Fixative
10% Formalin
Username

Mr. Jesse Wu

Verified customer

Submitted Oct 30 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
Sample
Human Tissue sections (Skin)
Specification
Skin
Permeabilization
Yes - 0.05% Tween 20
Fixative
10% Formalin
Username

Abcam user community

Verified customer

Submitted Oct 29 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6.0
Sample
Rabbit Tissue sections (Skin)
Specification
Skin
Permeabilization
Yes - 0.05% Tween 20
Fixative
10% Formalin
Username

Abcam user community

Verified customer

Submitted Oct 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse lung)
Specification
Mouse lung
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
Yes - Trion X-100
Blocking step
2% horse serum plus mouse Fab IgG fragment as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Mar 28 2013

Thank you for contacting us.
Attached are the two Certificates of Compliance you have requested.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products...

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The antibodies ab6401 and ab11214 are from the same clone [PCK-26], so they are the same antibody with similar binding characteristics. The difference is that ab6401 is not purified and is not conjugated while ab11...

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Thank you for contacting us.

I have found some references which support that the pan Cytokeratin and E Cadherin would be expressed by epidermal keratinocytes:

http://jmm.sgmjournals.org/content/56/7/971.full

http://www.uni...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (multiple tissues)
Loading amount
40 µg
Specification
multiple tissues
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Kris Frese

Verified customer

Submitted Jan 06 2012

Thank you for contacting us. The background should be black. I am sorry we never had a question like this before. It looks like this problem might be due to instrumentation then antibodies itself. I have following recommendations that might hel...

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