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Callus cytokeratins isolated from fresh human skin tissue
Our Abpromise guarantee covers the use of ab8068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/20. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration. Stains all types of keratin containing cells (epithelia) in frozen sections of various tissues, with the exception of myoepithelial cells.|
|IHC-P||Use at an assay dependent concentration. For paraffin embedded tissue a TUF pretreatment is recommended.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
ab8068 staining human foreskin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in 10mM Citrate buffer pH 6.0 in boiling water bath for 10 minutes, followed by cooling at room temperature for 30 minutes. The blocking was done using 1%BSA/5% normal donkey serum in PBS pH 7.4 for 2 hours at room temperature. The primary antibody, diluted 1/10 (PBS pH 7.4, 1%BSA, 0.1% sodium azide) and incubated with sample for 16 hours at 4°C. A HRP-conjugated donkey polyclonal to mouse Ig, diluted 1/1000 was used as the secondary.