Anti-pan Lamin antibody [X67, X167, X233] (ab20740)

Overview

  • Product name
    Anti-pan Lamin antibody [X67, X167, X233]
  • Description
    Mouse monoclonal [X67, X167, X233] to pan Lamin
  • Specificity
    Lamins do not appear to be universally distributed among different cell and tissue types. ab20740 recognizes the karyoskeleton, i.e. the intermediate filament proteins of the nucleus. Reacts with Lamin isotypes of Mr 60-75 kDa (LA, LI, LII, LIII, B1, B2, C). Other cell/tissue types have not been tested.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, Xenopus laevis, Kangaroo, Rainbow trout
  • Immunogen

    cell preparation: Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells).

Properties

Applications

Our Abpromise guarantee covers the use of ab20740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Used at a dilution of 1/50 for 16 hrs (see Abreview for further information). WB: Use at an assay dependent dilution. Predicted molecular weight: 60-75 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • ab20740 at a 1/50 dilution staining human U2 OS cells by Immunocytochemistry/Immunofluorescence. The cells were Paraformaldehyde fixed and incubated with the antibody for 16 hours. Bound antibody was detected using an Alexa- Fluor ® 488 conjugated goat anti-mouse IgG.

      The antibody was found to stain equally well when used at a dilution of 1/250.

      This image is courtesy of an Abreview submitted by Chi Tang on 6 April 2006.

      See Abreview

    • ab20740 staining pan Lamin in HeLa cells by Immunocytochemistry/ Immunofluorescence.
      Left image shows pan Lamin.
      Right image shows overlay of pan lamin (green), tubulin (red) and DNA (blue).

    References

    This product has been referenced in:
    • Azevedo AR  et al. Molecular Cytogenetics of Human Single Pronucleated Zygotes. Reprod Sci N/A:N/A (2014). Read more (PubMed: 24717739) »
    • Mackay DR  et al. Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint. J Cell Biol 191:923-31 (2010). ICC/IF ; Human . Read more (PubMed: 21098116) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Schmidtea mediterranea Tissue sections (whole animal)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 95 degree 15min citrate buffer pH6.0
    Permeabilization
    Yes - PBST 15min
    Specification
    whole animal
    Blocking step
    Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 28°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 05 2016

    Thank you for your enquiry and patience. This product is a mixture of 3 clones [X67, X167, X233] and two of the clones, X67 and X167, do react with Lamin C. I'm not able to obtain information at this time on exactly what samples were used to test this ...

    Read More

    Thank you for contacting me regarding the problems that you have experienced using this Ab. Where an Ab is giving non specific staining I would suggest decreasing the concentration at which the primary Ab is used at from 1/50 to 1/100 rather than us...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2 OS)
    Specification
    U2 OS
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5
    Username

    Dr. Chi W Tang

    Verified customer

    Submitted Apr 06 2006

    Thank you for your enquiry. This product is a mixture of 3 monoclonal antibodies, Clones X67, X167, and X223. The specific epitope locations have not been determined. The reactivity to lamin isotypes in different species for each clone is listed on t...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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