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Western blot - pan methyl Lysine (methyl K pan) antibody - ChIP Grade (ab7315)
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ChIP - pan methyl Lysine (methyl K pan) antibody - ChIP Grade (ab7315)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan methyl Lysine (methyl K pan) antibody - ChIP Grade (ab7315)
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Product Name
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pan methyl Lysine (methyl K pan) antibody - ChIP Grade
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to pan methyl Lysine (methyl K pan) - ChIP Grade
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Immunogen
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Purified calf thymus H1 chemically methylated on lysines. The reaction generates di-methyl lysine only.
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Reacts with
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Hu, At, Sc
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Predicted to react
(species key)
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Mamm
(Due to sequence homology)
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Specificity
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ab7315 recognises Histone H3 di-methyl K4, di-methyl K9 and to a lesser extent tri-methyl K27 in ELISA analysis.
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Tested applications
(see key)
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ChIP, ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP: Use at an assay dependent dilution. Every new batch of this antibody is tested at Abcam in ChIP.
ELISA: Use at an assay dependent dilution.
WB: 1/1000 - 1/2000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Histones >> H4 >> Methylated
Chromatin and Nuclear Signaling >> Histones >> H2A >> Methylated
Signal Transduction >> Metabolism >> Amino Acids
Chromatin and Nuclear Signaling >> Histones >> H3 >> Methylated
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Relevance
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Lysine methylation occurs in three distinct states, having either one (me1), two (me2) or three (me3) methyl groups attached to the amine group of the lysine side chain.
In eukaryotes, histone H3 trimethylated at lysine 4 (H3K4me3) is associated with active chromatin and gene expression.
This antibody will be extremely useful in the study of the regulation of transcription by methylation. Has also been successfully used in CHIP in both human and yeast.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this pan methyl Lysine (methyl K pan) antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for pan methyl Lysine (methyl K pan) antibody - ChIP Grade images, references, products related to ab7315 and other tools.
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pan methyl Lysine (methyl K pan) antibody - ChIP Grade images:
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 ChIP - pan methyl Lysine (methyl K pan) antibody - ChIP Grade (ab7315)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 6.5µl of ab7315 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - pan methyl Lysine (methyl K pan) antibody - ChIP Grade (ab7315)
IHC image of pan methyl Lysine (methyl K pan) staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7315, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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References for pan methyl Lysine (methyl K pan) antibody - ChIP Grade (ab7315)
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This product has been used in: (two most recent references)
Yang XD et al. Negative regulation of NF-kappaB action by Set9-mediated lysine methylation of the RelA subunit. EMBO J 28:1055-66 (2009). WB. PubMed: 19262565
Ea CK & Baltimore D Regulation of NF-kappaB activity through lysine monomethylation of p65. Proc Natl Acad Sci U S A 106:18972-7 (2009). ChIP; Human. PubMed: 19864627
See all 7 publication references for this product.
If you publish research using ab7315 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to pan methyl Lysine
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pan methyl Lysine (methyl K pan) antibody - ChIP Grade - more information
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