Overview

  • Product name
    Anti-pan Synuclein antibody
    See all pan Synuclein primary antibodies
  • Description
    Rabbit polyclonal to pan Synuclein
  • Specificity
    Detects endogenous levels of total Synuclein protein. This antibody recognises all forms of synuclein (alpha, beta and gamma).
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human pan Synuclein aa 15-64. The exact sequence is proprietary. Synthetic peptide derived from human synuclein.

  • Positive control
    • WB: Extracts from LOVO cells. IHC-P: Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab53726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • Relevance
    Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins: alpha, beta and gamma synucleins. Synuclein-alpha is primarily localized at the presynaptic region of axons and it may be involved in the regulation of dopamine release and transport. However, it can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Synuclein-alpha may induce fibrillization of the microtubule-associated protein tau and it reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. Synuclein-alpha is involved in Parkinson's disease, Lewy body dementia, Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome) and it is a major non-amyloid component of the deposits associated with Alzheimer's disease. Synuclein-beta may be a regulator of the SNCA aggregation process. It protects neurons from staurosporine and 6 hydroxy dopamine (6OHDA)-stimulated caspase activation that also involves p53 and contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Gamma synuclein is found primarily in the peripheral nervous system and retina. High levels of expression have been identified in advanced breast carcinomas suggesting a correlation between overexpression of SNCG and breast tumor development.
  • Cellular localization
    Neuronal cytosol and presynaptic terminals
  • Database links
  • Alternative names
    • BCSG1 antibody
    • beta synuclein antibody
    • Breast cancer specific gene 1 protein antibody
    • Gamma synuclein antibody
    • NACP antibody
    • PARK1 antibody
    • Persyn antibody
    • PRSN antibody
    • SNCA antibody
    • SNCB antibody
    • SNCG antibody
    • SR antibody
    • Synoretin antibody
    see all

Images

  • All lanes : Anti-pan Synuclein antibody (ab53726) at 1/500 dilution

    Lane 1 : rat heart lysate
    Lane 2 : rat heart lysate with immunizing peptide

    Lysates/proteins at 5 µg per lane.

    Secondary
    Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • Ab53726 at 1/50 dilution, staining paraffin embedded human brain in the presence (right) or absence (left) of blocking peptide.

References

This product has been referenced in:
  • Busch DJ  et al. Acute increase of a-synuclein inhibits synaptic vesicle recycling evoked during intense stimulation. Mol Biol Cell 25:3926-41 (2014). Read more (PubMed: 25273557) »
  • Lau BY  et al. Cyclic AMP promotes axon regeneration, lesion repair and neuronal survival in lampreys after spinal cord injury. Exp Neurol 250C:31-42 (2013). WB, IHC-P . Read more (PubMed: 24041988) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Lampreys (Petromyzon marinus) Tissue lysate - whole (Brain and spinal cord protein extracts)
Specification
Brain and spinal cord protein extracts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Jennifer Morgan

Verified customer

Submitted Feb 26 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Sample
Lampreys (Petromyzon marinus) Cell (spinal cord cryosections)
Specification
spinal cord cryosections
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde
Username

Jennifer Morgan

Verified customer

Submitted Feb 26 2015

Thank you for your enquiry. We are not aware of anyreferences for this antibody, and the only data we have is what is on the datasheet. You may want to consider the antibody ab6176. The datasheet lists six references.

http://www.abcam.com/ind...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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