Overview

  • Product nameAnti-pan Synuclein antibody
    See all pan Synuclein primary antibodies
  • Description
    Rabbit polyclonal to pan Synuclein
  • SpecificityThis antibody recognises all 3 forms of the synucleins.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human pan Synuclein aa 11-26. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC) and coupled to diphtheria toxoid.
    Sequence:

    AKEGVVAAAEKTKQGV

Properties

Applications

Our Abpromise guarantee covers the use of ab6176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
IHC-Fr 1/1000.
WB 1/1000.
ICC/IF Use a concentration of 1 µg/ml.

Target

Anti-pan Synuclein antibody images

  • ICC/IF image of ab6176 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6176, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-pan Synuclein antibody (ab6176)

This product has been referenced in:
  • Tenreiro S  et al. Yeast reveals similar molecular mechanisms underlying alpha- and beta-synuclein toxicity. Hum Mol Genet 25:275-90 (2016). Read more (PubMed: 26586132) »
  • Busch DJ & Morgan JR Synuclein accumulation is associated with cell-specific neuronal death after spinal cord injury. J Comp Neurol 520:1751-71 (2012). Read more (PubMed: 22120153) »

See all 8 Publications for this product

Product Wall

Application Western blot
Loading amount 2.5 µg
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Sample Lampreys (Petromyzon marinus) Recombinant protein (GST-tagged synucleins)
Specification GST-tagged synucleins
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Jennifer Morgan

Verified customer

Submitted Feb 27 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Sample Lampreys (Petromyzon marinus) Cell (Whole mounted lamprey brains)
Specification Whole mounted lamprey brains
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
Username

Jennifer Morgan

Verified customer

Submitted Feb 04 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Tissue lysate - whole (tectum)
Loading amount 20 µg
Specification tectum
Gel Running Conditions Reduced Denaturing (MINI-PROTEAN TGX)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. WANHUA SHEN

Verified customer

Submitted Apr 09 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"