Anti-Pancreatic alpha amylase antibody (ab21156)

Overview

  • Product name
    Anti-Pancreatic alpha amylase antibody
    See all Pancreatic alpha amylase primary antibodies
  • Description
    Rabbit polyclonal to Pancreatic alpha amylase
  • Specificity
    Cross-reactivity against other forms of amylase has not been determined.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Pancreatic alpha amylase.

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas

Properties

Applications

Our Abpromise guarantee covers the use of ab21156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000. Predicted molecular weight: 57 kDa. Strong reaction to 0.15ug and 0.3ug antigen detected. Predicted molecular weight: 57 kDa. Dilution optimised using Chromogenic detection.
IHC-P 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    Pancreatic amylase is a digestive enzyme secreted by the pancreas. It consists of a single polypeptide chain with a molecular weight of 54 kDa. Pancreatic amylase occurs in serum at a concentration of about 60µg/L, from a total amylase concentration in serum of about 120µg/L. Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis).
  • Cellular localization
    Secreted protein; extracellular space.
  • Database links
  • Alternative names
    • 1 4 alpha D glucan glucanohydrolase antibody
    • 1,4 alpha D glucan glucanohydrolase 2B antibody
    • Alpha amylase 2B antibody
    • Alpha amylase antibody
    • AMY2 antibody
    • AMY2A antibody
    • AMY2B antibody
    • AMY3 antibody
    • Amylase alpha 2A antibody
    • Amylase alpha 2A pancreatic antibody
    • Amylase alpha 2B antibody
    • Amylase alpha 2B pancreatic antibody
    • Amylase pancreatic alpha 2A antibody
    • Amylase pancreatic alpha 2B antibody
    • Amylase, alpha 2A (pancreatic) antibody
    • Amylase, alpha 2B (pancreatic) antibody
    • Carcinoid alpha amylase antibody
    • Found in the pancreas antibody
    • Glycogenase antibody
    • HXA antibody
    • PA antibody
    • Pancreatic alpha amylase antibody
    • Pancreatic amylase 2A antibody
    • Pancreatic amylase alpha 2A antibody
    see all

Anti-Pancreatic alpha amylase antibody images

  • IHC image of Pancreatic Amylase staining in Human pancreas normal formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21156, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Pancreatic alpha amylase antibody (ab21156)

This product has been referenced in:
  • Shiokawa M  et al. Pathogenicity of IgG in patients with IgG4-related disease. Gut N/A:N/A (2016). Human . Read more (PubMed: 26964842) »
  • Khoriaty R  et al. Pancreatic SEC23B deficiency is sufficient to explain the perinatal lethality of germline SEC23B deficiency in mice. Sci Rep 6:27802 (2016). IHC . Read more (PubMed: 27297878) »

See all 3 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Permeabilization
No
Fixative
no
Username

Mrs. Sandy Piampiano

Verified customer

Submitted Feb 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid buffer
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Serum (serum)
Loading amount
30 µg
Specification
serum
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 24 2012

Thank you for your inquiry.

Unfortunately, I am unable to issue discount code for the following antibodies:

ab60720, ab86704 and ab65109.

The immunogens for the MUC antibodies do not show a high enough homology (70% and be...

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Thank you for contacting us. Generally we predict reactivity of an antibody if the immunogen sequence has 85% or greater homology with the species being tested. The immunogen for ab35414 and ab82890 is full length pig protein and for ab21156 it is full...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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