Anti-Pancreatic Polypeptide antibody (ab77192)

Overview

  • Product name
    Anti-Pancreatic Polypeptide antibody
    See all Pancreatic Polypeptide primary antibodies
  • Description
    Goat polyclonal to Pancreatic Polypeptide
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide:

    TRPRYGKRHKEDT

    , corresponding to amino acids 61-73 of Human Pancreatic Polypeptide

  • Positive control
    • Human pancreas

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 10 kDa).

Preliminary experiments gave an approx 35-38 kDa band in human pancreas lysate, which was successfully blocked by incubation with the immunizing peptide.  Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa.

 

ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:32,000 by peptide ELISA.

IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans.

ICC/IF 1/150.

See Abreview

Target

  • Relevance
    Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Pancreatic polypeptide antibody
    • Pancreatic polypeptide Y antibody
    • Pancreatic prohormone antibody
    • PNP antibody
    • PP antibody
    • PPY antibody
    • prepro-PP (prepropancreatic polypeptide) antibody
    see all

Images

  • Staining of formalin-fixed, paraffin-embedded Human pancreas tissue with 3µg/ml ab77192. Note strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans.

References

This product has been referenced in:
  • Ackermann AM  et al. Integration of ATAC-seq and RNA-seq identifies human alpha cell and beta cell signature genes. Mol Metab 5:233-44 (2016). Human . Read more (PubMed: 26977395) »
  • Wang YJ  et al. Single-Cell Mass Cytometry Analysis of the Human Endocrine Pancreas. Cell Metab 24:616-626 (2016). Alexa Fluor® 568 ; Human . Read more (PubMed: 27732837) »

See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer pH6.0
Specification
Pancreas
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rm°C
Fixative
Formaldehyde
Username

Jing Ma

Verified customer

Submitted Mar 28 2016

Thank you for contacting us.

Unfortunately this lot is sold out so we can not offer this - we however have new lot in sock.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Nov 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Pancreas)
Specification
Pancreas
Fixative
Paraformaldehyde
Permeabilization
Yes - PBSx1/ 0.1% TritonX100
Blocking step
No blocking step used for 30 minute(s) · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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