The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/500 - 1/1000. Detects band of approximately 37 and 50 kDa in two separate blots (predicted molecular weight for isoform 2: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a role in the physiological regulation of the intracellular CoA concentration.
Tissue specificityIsoform 1 is expressed at high levels in brain, heart, kidney, liver, skeletal muscle and testis. Isoform 2 is detected at much lower levels in kidney, liver, brain and testis and is not detected in heart or skeletal muscle.
PathwayCofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.
Sequence similaritiesBelongs to the type II pantothenate kinase family.
DomainThe N-terminal extension, present in isoform 1 may be the regulatory domain.