Overview

  • Product name
  • Description
    Mouse polyclonal to PANK1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human PANK1 protein (NP_683879.1)

  • Positive control
    • PANK1 transfected 293T cell lysate and human liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects band of approximately 37 and 50 kDa in two separate blots (predicted molecular weight for isoform 2: 41 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Plays a role in the physiological regulation of the intracellular CoA concentration.
    • Tissue specificity
      Isoform 1 is expressed at high levels in brain, heart, kidney, liver, skeletal muscle and testis. Isoform 2 is detected at much lower levels in kidney, liver, brain and testis and is not detected in heart or skeletal muscle.
    • Pathway
      Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.
    • Sequence similarities
      Belongs to the type II pantothenate kinase family.
    • Domain
      The N-terminal extension, present in isoform 1 may be the regulatory domain.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • coaA antibody
      • hPanK antibody
      • hPanK1 antibody
      • PANK antibody
      • PANK1 antibody
      • PANK1_HUMAN antibody
      • PANK1a antibody
      • PANK1b antibody
      • PanK1beta antibody
      • Pantothenate kinase 1 antibody
      • Pantothenate kinase antibody
      • Pantothenic acid kinase 1 antibody
      see all

    Images

    • Anti-PANK1 antibody (ab69354) at 1/500 dilution + human liver tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 41 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-PANK1 antibody (ab69354) at 1/500 dilution

      Lane 1 : PANK1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 41 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    References

    ab69354 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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