Overview

  • Product nameAnti-PANK1 antibody
    See all PANK1 primary antibodies
  • Description
    Mouse polyclonal to PANK1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human PANK1 protein (NP_683879.1)

  • Positive control
    • PANK1 transfected 293T cell lysate and human liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects band of approximately 37 and 50 kDa in two separate blots (predicted molecular weight for isoform 2: 41 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a role in the physiological regulation of the intracellular CoA concentration.
    • Tissue specificityIsoform 1 is expressed at high levels in brain, heart, kidney, liver, skeletal muscle and testis. Isoform 2 is detected at much lower levels in kidney, liver, brain and testis and is not detected in heart or skeletal muscle.
    • PathwayCofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.
    • Sequence similaritiesBelongs to the type II pantothenate kinase family.
    • DomainThe N-terminal extension, present in isoform 1 may be the regulatory domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • coaA antibody
      • hPanK antibody
      • hPanK1 antibody
      • PANK antibody
      • PANK1 antibody
      • PANK1_HUMAN antibody
      • PANK1a antibody
      • PANK1b antibody
      • PanK1beta antibody
      • Pantothenate kinase 1 antibody
      • Pantothenate kinase antibody
      • Pantothenic acid kinase 1 antibody
      see all

    Anti-PANK1 antibody images

    • Anti-PANK1 antibody (ab69354) at 1/500 dilution + human liver tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 41 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-PANK1 antibody (ab69354) at 1/500 dilution

      Lane 1 : PANK1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 41 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    References for Anti-PANK1 antibody (ab69354)

    ab69354 has not yet been referenced specifically in any publications.

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