This antibody gave a positive signal in Human Brain tissue lysate.
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ICC/IF image of ab124131 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab124131, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HepG2 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa and HepG2 cells at 1µg/ml
Sana-Ur-Rehman H et al. Expression and localization of pannexin-1 and CALHM1 in porcine bladder and their involvement in modulating ATP release. Am J Physiol Regul Integr Comp Physiol312:R763-R772 (2017).
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Koyanagi S et al. Glucocorticoid regulation of ATP release from spinal astrocytes underlies diurnal exacerbation of neuropathic mechanical allodynia. Nat Commun7:13102 (2016).
Read more (PubMed: 27739425) »