Anti-Pannexin 1 antibody (ab124131)
Key features and details
- Rabbit polyclonal to Pannexin 1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Pannexin 1 antibody
See all Pannexin 1 primary antibodies -
Description
Rabbit polyclonal to Pannexin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Pig, Macaque monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Pannexin 1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab124143) -
Positive control
- WB: Human brain tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
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IHC-P |
1/1000. PubMed: 24269631
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). |
IHC-P
1/1000. PubMed: 24269631 |
Target
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Function
Structural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis. -
Sequence similarities
Belongs to the pannexin family. -
Cellular localization
Cell membrane. Cell junction > gap junction. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 24145 Human
- Entrez Gene: 55991 Mouse
- Entrez Gene: 100171730 Orangutan
- Entrez Gene: 315435 Rat
- Omim: 608420 Human
- SwissProt: Q96RD7 Human
- SwissProt: Q9JIP4 Mouse
- SwissProt: Q5REE3 Orangutan
see all -
Alternative names
- innexin antibody
- MRS1 antibody
- Pannexin 1 antibody
see all
Images
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Anti-Pannexin 1 antibody (ab124131) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
The predicted molecular weight of Pannexin 1 is 48 kDa (SwissProt), however we expect to observe a banding pattern at 45 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Protocols
Datasheets and documents
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Datasheet download
References (14)
ab124131 has been referenced in 14 publications.
- Zheng Y et al. Probenecid-Blocked Pannexin-1 Channel Protects Against Early Brain Injury via Inhibiting Neuronal AIM2 Inflammasome Activation After Subarachnoid Hemorrhage. Front Neurol 13:854671 (2022). PubMed: 35401398
- Wang Q et al. Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses. J Neuroinflammation 19:244 (2022). PubMed: 36195881
- Li S et al. Carbenoxolone inhibits mechanical stress-induced osteogenic differentiation of mesenchymal stem cells by regulating p38 MAPK phosphorylation. Exp Ther Med 15:2798-2803 (2018). PubMed: 29456683
- Zhou L et al. Pannexin-1 is involved in neuronal apoptosis and degeneration in experimental intracerebral hemorrhage in rats. Mol Med Rep 17:5684-5691 (2018). PubMed: 29484398
- Eguchi R et al. Fibroblast growth factor 2 modulates extracellular purine metabolism by upregulating ecto-5'-nucleotidase and adenosine deaminase in cultured rat spinal cord astrocytes. J Pharmacol Sci N/A:N/A (2018). PubMed: 30598426