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Rabbit polyclonal to Pannexin 2
Predicted to work with:
Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
A region within synthetic peptide: NFAEEPIYCY TPHNFTRDQA LYARGYCWTE LRDALPGVDA SLWPSLFEHK, corresponding to N terminal amino acids 73-122 of Human Pannexin 2
HepG2 cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).Can be blocked with
Pannexin 2 peptide (ab190829). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Structural component of the gap junctions and the hemichannels.
Belongs to the pannexin family.
Cell membrane. Cell junction > gap junction.
Information by UniProt
Pannexin 2 antibody
Western blot - Pannexin 2 antibody (ab55917)
Anti-Pannexin 2 antibody (ab55917) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size: 69 kDa Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Pannexin 2 antibody(ab55917)
Paraffin embedded human muscle tissue incubated with ab55917 at 8µg/ml. Positively labelled skeletal muscle cells are indicated with arrows. Magnification: 400X
Western blot - Anti-Pannexin 2 antibody (ab55917)
All lanes : Anti-Pannexin 2 antibody (ab55917) at 2.5 µg/ml Lane 1 : HepG2 cell lysate Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml Lysates/proteins at 25 µg per lane. Predicted band size: 69 kDa
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