Anti-Pannexin 2 antibody [N121A/31] (ab167442)

Overview

  • Product name
    Anti-Pannexin 2 antibody [N121A/31]
    See all Pannexin 2 primary antibodies
  • Description
    Mouse monoclonal [N121A/31] to Pannexin 2
  • Host species
    Mouse
  • Specificity
    ab167442 does not cross-react with Pannexin 1 or Pannexin 3.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse
  • Immunogen

    Fusion protein, corresponding to amino acids 11-664 of Rat Pannexin 2 (UniProt P60571).

  • Positive control
    • WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
  • General notes

    The clone number has been updated from S121A-31 to N121A/31, both clone numbers name the same antibody clone.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    N121A/31
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 74 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/100.

Target

Images

  • 4% formaldehyde-fixed SK-N-BE cells labeling Pannexin 2 using ab167442 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/200 dilution incubated for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain (red). Nuclei were stained with DAPI (blue).

  • Anti-Pannexin 2 antibody [N121A/31] (ab167442) at 1/200 dilution + Rat brain membrane lysate at 15 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 74 kDa

References

ab167442 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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