Overview

  • Product nameAnti-PAOX antibody
    See all PAOX primary antibodies
  • Description
    Rabbit polyclonal to PAOX
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to a region of Human PAOX

  • Positive control
    • Human fetal muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab75199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • RelevancePAOX is a flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. It can also oxidize N(1)-acetylspermidine to putrescine. It does not oxidize spermidine. PAOX plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. There are eleven named isoforms.
  • Cellular localizationPeroxisome. Cytoplasm.
  • Database links
  • Alternative names
    • DKFZp434J245 antibody
    • MGC45464 antibody
    • PAO antibody
    • Peroxisomal N(1) acetyl spermine/spermidine oxidase antibody
    • Peroxisomal N1 acetyl spermine/spermidine oxidase antibody
    • Polyamine oxidase (exo N4 amino) antibody
    • Polyamine oxidase antibody
    • RP11 122K13.11 antibody
    see all

Anti-PAOX antibody images

  • Anti-PAOX antibody (ab75199) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa

References for Anti-PAOX antibody (ab75199)

ab75199 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"